Anti-5 methylcytosine monoclonal antibodies

Anti-5 methylcytosine monoclonal antibodies
PUBLICATIONS
1985-1990
Adouard, V., Dante, R., Niveleau, A., Revet, B., Delain, E. and Ehrlich, M. (1985)
The accessibility of 5-methylcytosine to specific antibodies in double-stranded DNA of
Xanthomonas phage XP12 Europ. J. Biochem. 152 115-121
C. Reynaud, A. Niveleau 1(990)
Methylated bases as tumor markers.: Detection and quantitation with a competitive enzyme-linked
immunoassay. Anal. Letters 23, 31-45
C. Reynaud, C. Bruno, P. Boullanger, J. Grange, S. Barbesti and A. Niveleau.
Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six
monoclonal antibodies Cancer Letters (1991) 61 255-262
1991-1995
R. Dante, A. Baldini, D.A. Miller, M. Rocchi, A. Niveleau and O.J. Miller. (1991)
Methylation des régions 5' promotrices de l'ARN ribosomal humain dans des lignées
lymphoblastoides et un hybride somatique humain-hamster. in Chromatine Aspects structuraux et
fonctionnels. Editions du CNRS 297-305
C. Reynaud, C. Bruno, P. Estrabaud, S. Barbesti and A. Niveleau. (1992)
Monitoring the excretion of modified nucleosides by cancer patients with a six-monoclonal antibody
set in a solid-phase immunoassay. Amer. Assoc. Cancer Research. 33, 201.(Abstarct)
R. Dante, A. Baldini, D.A. Miller, M. Rocchi and A. Niveleau. (1992)
Methylation of the 5' flanking sequences of the ribosomal DNA in human cell lines and in a human-
hamster hybrid cell line. J. Cell. Biochem. 50, 357-362.
C. Reynaud, L. Frappart and A. Niveleau (1994)
Immunochemical detection of modified nucleosides in DNA and RNA in cells grown in vitro and in
breast tissues 85th Annual Meeting. Amer. Ass. Cancer Research 35, 15 (Abstract)
Montpellier, C. Bourgeois, C., Kokalj-Vokac, N., Muleris,M. ,Niveleau, A. , Gibault, A.,
Malfoy, B. and Dutrillaux,B. (1994
Detection of methylcytosine rich heterochromatin on banded chromosomes . Application to cells
with various status of DNA methylation. Cancer Genetics Cytogenetics. 78, 87-93
Barbin A., Montpellier,C., Kokalj-Vokac, N.,Gibaud,A., Niveleau, A., Dutrillaux, B.,
Malfoy, B. and Bourgeois C.A. (1994)
In situ DNA methylation analysis of human tumor cells Mapping of methylcytosine-rich bands.
Human Genetics, 94, 684-692
Niveleau, A., Drouet, C., Bruno, C. Reynaud, , C., Fares, F. (1994)
Polymerase chain reaction products containing 5-methylcytidine. A microplate
immunoquantification method. Nucleic Acids Res. 22, 5508-5509
Delain,E., Barbin-Arbogast,A., Bourgeois,C., Mathis,G., Moryd,C., Favard,C. and
Niveleau,A.. (1995)

The limits in electron microcopy of macromolecules interactions. The use of new labels based on
lanthanide cryptates. An interdisciplinary approach.Journal of Trace and Microprobes Techniques,
13(3), 371-381
Miniou,P., Jeanpierre,M., Blanquet,V.,Sibella,V., Bonneau,D., Herbelin,C., Fischer,A.,
Niveleau,A. & Viegas-Péquignot,E. (1994)
Abnormal methylation pattern in constitutive and facultative (X inactive chromosome)
heterochromatin of ICF patients. Human Mol. Genetics 3, 2093-2102
Veilleux,C., Bernardino,J., Gibaud,A.,Niveleau,A., Malfoy,B., Dutrillaux,B. et Bourgeois,C.
(1995)
Alterations de la methylation dans les cellules tumorales: approche quantitative "in situ". Bull
Cancer 82, 939-945
1996-2000
de Capoa, A., Menendez,F., Poggesi, I., Grapelli, C., Marotta, M.R., Di Leandro, M.,
Reynaud, C., Niveleau, A. (1996)
Cytological evidence for 5-azacytidine-induced demethylation of the heterochromatic regions of
human chromosomes Chromosome Research, 4,271-276.
Bernardino,J., Lamoliatte,E., Lombard,M., Niveleau,A., Malfoy,B., Dutrillaux,B.,
Bourgeois,C.A. (1996)
DNA methylation of the X chromosomes of the human female: an in situ semi-quantitative
analysis. Chromosoma, 104: 528-535.
Habib, M., Bourgeois, C.A., Truong,K., Bernardino, J., Dutrillaux, B., Souchier, C.,
Niveleau, A. (1996)
DNA methylation and cancer: an in situ approach. Proceedings American Association against
Cancer. 37, 535 (abstract)
Sasco, A.J., Rey, F., Reynaud, C., Bobin, J.Y., Clave, M. , Niveleau, A. (1996)
Breast cancer prognostic significance of some modified urinary nucleosides. Cancer Letters, 108,
157-162.
Tweedie, S., Charlton, J., Clark, V., Bird, A. (1997).
Methylation of genomes and genes at the invertebrate-vertebrate boundary. Molecular and cancer
biology 17: 1469-1475.
Bensaada, M., Kiefer, H., Tachdjian,G., Lapierre, J.-M., Cacheux, V., Niveleau, A., Métézeau, P.
(1998)
Altered paterns of DNA methylation on chromosomes from leukemia cell lines.Identification of 5-
methylcytosines by indirect immunodetection. Cancer Genet Cell Genet. 103: 101-109.
Tebib, J.G., Reynaud, C., Cedoz, J.P., Letroublon, M.C., Niveleau, A. (1997).
Relationship between urinary excretion of modified nucleosides and rheumatoid arthritis process.
Br. J. Rheumatol. 36, 990-995.
Kokalj-Vokac N., Zagorac, A., Pristovnik, M., Bourgeois, C.A., Dutrillaux, B. (1998)
DNA methylation of the extraembryonic tissues: an in situ study on human metaphase
chromosomes. Chromosome Res, 6: 161-166.
de Capoa A, Di Leandro M,Grappelli C, Menendez F, Poggesi I, Giancotti P, Marotta MR, Spanò A,
Rocchi M, Archidiacono N, Niveleau A (1998)
Methylation status of individual interphase nuclei in human cultured cells: A semiquantitative
computerised analysis. Cytometry, 31, 85-92.

Habib, M., Fares, F., Drouet, E., Niveleau, A. (1998)
DNA methyltransferase activity and DNA methylation levels: evaluation with a non-radioactive
technique. Proceedings 89 th Annual Meeting AACR.39, 94 (Selected Abstract)
Rougier, N., Bourch’is, D., Molina, D., Niveleau, A., Plachot, M., Paldi, A., Viegas-Pequignot, E. (1998)
Chromosome methylation patterns during mammalian pre-implantation development. Genes and
Dev. 14, 2108-2113.
Benchaib, M., Ajina, M., Souchier, C., Niveleau, A., Lornage, J., Guerin, J.-F. (1998)
A protocol to investigate DNA methylation staining by image analysis in human spermatozoa.. In
Genetics of human male fertility. C Barratt, C. De Jonge, D. Mortimer, J. Parinaud. Eds. EDK 1997
222-231.
de Capoa, A., Romana Febbo, F., Giovanelli, F., Niveleau, A., Zardo, G., Marenzi, S., Caiafa, P.
(1999)
Reduced levels of Poly(ADP)-ribosylation result in chromatin compaction and hypermethylation
as shown by a cell-by-cell computer-assited quantitative analysis. FASEB. J. 13, 89-93.
Habib, M., Fares, Bella, C., F., Bourgeois,C.A., Bernardino,J., Blazquez-Hernandez, F., de Capoa A,
Niveleau, A. (1999)
DNA global hypomethylation in EBV-transformed interphase nuclei. Exp. Cell Res. 249, 46-53
de Capoa, A., Grappelli, C., Febbo, F.R, Spano, A., Niveleau, A., Cafolla, A., Cordone, I., Foa, A.
(1999)
Methylation levels of normal and chronic lymphocytic leukemia B lymphocytes: computer-assisted
quantitative analysis of anti-5-methylcytosine antibody binding to individual nuclei. Cytometry, 36,
157-159
Xu, G.-L., Bestor, T.H., Bourc’his, D., Hsieh, C.-L., Tommerup, N., Bugge, M., Hulten, M., Qu, X.,
Russo, J.J., Viegas-Pequignot, E. (1999)
Chromosome instability and immunodeficiency syndrome caused by mutations in a DNA
methyltransferase gene. Nature, 402: 187-191.
Mayer, W., Niveleau, A., Walter, J., Fundele, R., Haaf, T. (2000)
Active demethylation of the zygotic paternal genome. Nature 403: 501-502.
Coffigny, H., Bourgeois, C., Ricoul, M., Bernardino, J., Vilain, A., Niveleau, A., Malfoy, B. Dutrillaux, B.
(1999)
Alterations of DNA methylation patterns in germ and Sertoli cells from developing mouse testis.
Cytogenet. Cell. Genet. 87: 175-181.
Stancheva, I. and Meehan, R.M. (2000)
Transient depletion of xDnmt1 leads to premature gene activatioon in Xenopus embryos. Genes
and Development, 14: 313-327.
Bernardino J, Lombard M, Niveleau A, Dutrillaux B (2000).
Common methylation characteristics of sex chromosomes in somatic and germ cells
from mouse, lemur and human. Chromosome Res.;8(6):513-25.
F.J. Hernandez-Blazquez, M. Habib, J.-M. Dumollard, C. Barthelemy, M. Benchaib , A. de Capoa, A.
Niveleau. (2000)
Evaluation of global DNA hypomethylation in human colon cancer tissues by
immunohistochemistry and image analysis. Gut 47: 689-693.
Vilain A, Bernardino J, Gerbault-Seureau M, Vogt N, Niveleau A, Lefrancois D, Malfoy B, Dutrillaux B.
(2000)
DNA methylation and chromosome instability in lymphoblastoid cell lines.
Cytogenet Cell Genet.;90(1-2):93-101.

Zagradisnik B, Kokalj-Vokac N. (2000)
Hypomethylation of alphoid DNA and classical satellite DNA on chromosome 1, 9,
16 and Y in extraembryonic tissue. Pflugers Arch.;440(5 Suppl):R190-2.
2001-
Taddei, A., Maison, C., Roche, D., Almouzni, G. (2001)
Reversible disruption of pericentric heterochromatin and centromere function by inhibiting
deacetylases in mammalian cells. Nature Cell Biol, 3 :114-120
Piyathilake CJ, Johanning GL, Frost AR, Whiteside MA, Manne U, Grizzle WE, Heimburger DC,
Niveleau A. (2000)
Immunohistochemical evaluation of global DNA methylation: comparison with in vitro radiolabeled
methyl incorporation assay. Biotech Histochem. 2000 Nov;75(6):251-8.
F. Grützner, E. Zend-Ajusch, K. Stout, S. Munsche, A. Niveleau, I. Nanda, M. Schmid, and T.
Haaf (2001)
Chicken microchromosomes are hypermethylated and can be identified by specific painting
probes Cytogenet. Cell Genet., 93:265-269.
Piyathilake CJ, Frost AR, Bell WC, Oelschlager D, Weiss H, Johanning GL, Niveleau A, Heimburger
DC, Grizzle WE. (2001)
Altered global methylation of DNA: An epigenetic difference in susceptibility for lung cancer is
associated with its progression. Human Pathol. 32(8):856-62
Bourch’is, D., Le Bourhis, D., Patin, D., Niveleau, A., Comizzoli, P., Renard, J.P., Viegas-Pequignot,
E. (2001).
Delayed and incomplete reprogramming of chromosome methylation patterns in bovine cloned
embryos. Current biology, 11:1542-1546
Barton, S.C., Arney, K.L., Shi, W., Niveleau, A., Fundele, R., Surani, M.A., Haaf, T. (2001)
Genome-wide methylation patterns in normal and uniparental early mouse embryos. Human Mol.
Genetics. 10:2983-2987
Stancheva, I., El-Maarri, O., Walter, J., Niveleau,A. Meehan, R.R. (2002).
DNA methylation at promoter regions regulate the timing of gene activation in Xaenopus laevis
embryos. Dev. Biol. 243:155-165

COMMUNICATIONS
A. Niveleau, C. Reynaud and J. Grange
Urinary excretion of non protein tumor markers: monitoring by EIA. Journées immunologiques.
Fondation franco-allemande. Paris 24-26 Novembre 1988
Niveleau, A.
Bases puriques et pyrimidiques modifiées. 6° Symposium Biochimie Clinique des Cancers.
Fondation Bergonié. 27-29 Avril 1989 Bordeaux.
Dante, R., Baldini, A., Miller, D.A., Rocchi, M. and Niveleau, A.
Methylation du promoteur du rDNA humain dans les lymphocytes humains et des hybrides
somatiques. Forum des jeunes chercheurs. Juin 1990 Lyon.
Reynaud, C., Bruno, C., Niveleau, A., Boullanger, P., et J Grange J.
Dosage immunoenzymatique à l'aide d'anticorps monoclonaux de bases modifiées dans les
liquides biologiques. Colloque régional INSERM. 17-19 Octobre 1990.Lyon.
L. Frappart, C. Reynaud, C. Bruno and A. Niveleau
Modified nucleosides in malignancy. Detection, visualization and quantification in human
tissues and urine with a set of monoclonal antibodies. European Workshop on genetic
alterations in human solid tumors. 9-11 Mai 1991 Montpellier
C. Reynaud, C. Bruno and A. Niveleau
Les nucléosides modifiés en cancérologie clinique Dosage de l'excrétion urinaire au moyen
de six anticorps monoclonaux. Optimisation des essai. Etude statistique sur 200 cas. VII°
Symposium Biochimie Clinique des Cancers Deauville 15-16 Mai 1991
L. Frappart, C. Pechoux, C. Reynaud et A. Niveleau
Les nucléosides modifiés en cancérologie clinique. Etude immunocytochimique de
l'accessibilité de la 5-methylcytidine et de la pseudo-uridine dans les lésions bénignes et
malignes du sein. VII° Symposium Biochimie Clinique des Cancers. Deauville 15-16 Mai.
S. Barbesti, A. Niveleau, C. Reynaud and P. Giordano.
A new enzyme immunoassay for modified nucleosides. 25 Years of immunoenzymatic
techniques International Congress Athens September 9-12.1991
C. Reynaud, P. Bruno, P. Boullanger, J. Grange, S. Barbesti and A. Niveleau
Excretion of modified nucleosides by cancer patients: monitoring by EIA. Tumor markers:
current applications and impact on Therapy. 8th International Conference of IATMO. Nice 16-19
Novembre 1991.
S. Barbesti, A. Niveleau, C. Reynaud, C. Bruno, M. Danova and P. Giordano.
Monoclonal antibodies for new tumor marker immunoassay tests. International Symposium
"New generation of monoclonal antibodies in diagnosis and therapy" Gênes (Italie) 12-15 Avril
1992
C. Reynaud, P. Estrabaud , E. Archimbaud, A. Perret-Liaudet, P. Later,and A. Niveleau.
Monitoring the excretion of modified nucleosides in haematological malignancies. Conference
on Anticancer Research. Crête, Grèce. Octobre 1992
L. Frappart , C. Reynaud, C. Pechoux ,and A. NIVELEAU.
Modified nucleosides in clinical oncology: Studies on the accessibility of 5-methylcytidine and
pseudouridine-containing macromolecules to monoclonal antibodies in breast tumors. Fourth
International Conference on Anticancer Resarch. Crête, Grèce. Octobre 1992
A. Niveleau. C. Reynaud and C. Bruno.
Non-protein tumor markers:modified nucleosides. 17 th Congress of the European Society for
Medical Oncology. Lyon. France. Novembre 1992.