annee 2015-‐16

!
!
!
!
"#$%&'!(!'&)*&')*&!+,-&)%+./.0+&!-.,1#"&,%#/&!
#,,&&!(234536!
!
!
%789:!;<!=<>:8!;:!=8?@:A!!
!"#$%&$%&'()**#+)",&)++,%&$-+.&'-&/,01+.%&-+")2)+3%4")%#.%&5&$%.4/)0")1+&$%&'-&6&"/-)+%$&)++-"%&)**#+)"7&8&
$-+.&'%.&%.094%.&:,",/)+-)/%.&
!
!
,BCD!?;9:==:!;:!EFGH78I!;F?JJ<:7E!K!,BC!;<!9:=LBH=?ME:!;:!EFGH78IA!!
;!<=>?&&
?-@1/-"1)/%&$(=**#+1'1A)%&&
?-@1/-"1)/%&'71+&B%/'-+$&
CDEF&/#%&;-/4%'&;,/)%#G&
HIJJK&?LMN&
<%.01+.-@'%&$%&'(#+)",&5&O/&P-/%''%&O%&?#4-
! ! !!!!!
!
,BCD!?;9:==:!;:!EF&N<7L:!;F?JJ<:7EK!,BCD!8:ED!?;9:==:!:5C?7E!;<!9:=LBH=?ME:!;F&N<7L:!O!JBH8?J8:9A!
;!<=>?&
?-@1/-"1)/%&$(=**#+1'1A)%&&
?-@1/-"1)/%&'71+&B%/'-+$&
CDEF&/#%&;-/4%'&;,/)%#G&
HIJJK&?LMN&
O/&P-/%''%&O%&?#4- (JEKCKCQCKIF&P-/%''%R$%'#4-S*%/)-'R41*T&
!
,BC!;:!EF&HJ?;9?H8D!=7!;7PPI9:H8!;<!9:=LBH=?ME:!;FIN<7L:!
?#$):)+%&UV-0-"&
?#$):)+%RUV-0-"S*%/)-'R41*&
!
'I=<CI!;<!$<>:8!;:!=8?@:A!!
"#$!%&'&()*#$!$&'*!+#$!(#,,-,#$!(,.$!+-!$)$*/%#!0%%-'0*102#!0''.!3-0!412*0(04#'*!5!,1!42#%0/2#!,06'#!+#!
+.7#'$#!(&'*2#!,#$!0'7#(*0&'$!#*!10+#'*!5!0'0*0#2!,1!2.4&'$#!1+14*1*08#!3-0!#$*!$4.(0703-#!+-!41*9&6/'#!#*!
4&$$/+#!+#$!(141(0*.$!#77#(*20(#$!#*!%.%&02#$:!
;,!1!2.(#%%#'*!.*.!+.%&'*2.!(9#<!,1!$&-20$!#*!(9#<!,=9&%%#!3-#!,#$!%&'&()*#$!4#-8#'*!>*2#!#'*210'.$!,&2$!
+=-'#!0'7#(*0&'!420%102#!5!2.1602!(&'*2#!+#$!0'7#(*0&'$!$#(&'+102#$!+#!%1'0/2#!4,-$!0'*#'$#:!"&0'!+=>*2#!-'!
%.(1'0$%#!+#!%.%&02#!$4.(0703-#!+=-'!1'*06/'#?!,1!(&%%-'1-*.!$(0#'*0703-#!412,#!+=!@!#'*210'#%#'*!A:!
B#*!#'*210'#%#'*!+#!,=0%%-'0*.!0''.#!4#-*?!#'!1C$#'(#!+#!$)$*/%#!1+14*1*07?!42&*.6#2!+#$!$&-20$!(&'*2#!
+#$!0'7#(*0&'$!$#(&'+102#$!9.*.2&,&6-#$:!
B9#<!,=9&%%#?!142/$!-'#!81((0'1*0&'!DBE?!(#$!%&'&()*#$!#'*210'.$!42&+-0$#'*!+#$!*1-F!+#!()*&G0'#$!42&H
0'7,1%%1*&02#$!$-4.20#-2$!1-F!8&,&'*102#$!$10'$!'&'!81((0'.$!,&2$3-=0,$!$&'*!%0$!#'!(&'*1(*!18#(!+=1-*2#$!
41*9&6/'#$!ex#vivo:!B#$!(#,,-,#$!4#-8#'*!$#!%10'*#'02!3-#,3-#$!%&0$!'&*1%%#'*!412!+#$!%.(1'0$%#$!+#!
2#42&621%%1*0&'$!.406.'.*03-#$!#*!%.*1C&,03-#$:!
!
I&*2#!,1C&21*&02#!$#!42&4&$#!+=.*-+0#2!$#$!'&-8#1-F!%.(1'0$%#$!+#!42&*#(*0&'!+#!,=0%%-'0*.!0''.#!(&'*2#!
+#$!0'7#(*0&'$?!4&-81'*!$=144120#2!5!-'#!%.%&02#!0%%-'0*102#!0''.#!'&'!$4.(0703-#?!+1'$!+#$!#$4/(#$!
(0C,#$!+#!,1!81((0'1*0&'!8.*.20'102#:!

J!412*02!+=.(91'*0,,&'$!$1'6-0'$!+=1'0%1-F!'1K7$!&-!81((0'.$?!0,!$=16021!+#!+.%&'*2#2!,#!42&70,!+=#F42#$$0&'!
()*&G0'03-#!+#$!%&'&()*#$!142/$!2#'(&'*2#!!18#(!-'!$*0%-,-$!in(vitro:!"#$!%&+070(1*0&'$!.406.'.*03-#$!
$#2&'*!0'8#$*06-.#$:!"#!$-080!+#!,1!2.4&'$#!0%%-'0*102#!,&2$!+#!42&*&(&,#$!(,0'03-#$!$#21!.61,#%#'*!0'*.62.!
1-!$*16#:!
!
!
%:JQHBEB@7:=!<87E7=I:=A!!
Culture(cellulaire(:(culture(de(cellules(primaires,(de(tissus,(isolement(de(population(cellulaire(par(tri(
magnétique(
Immunologie(:(Cytométrie(en(flux,(Luminex,(ELISA,(ELISPOT(
Biochimie(:(Western(Blot(
Biologie(Moléculaire(:(PCR#Q(
Statistiques(
!
!
"B8=!JEI=!A!7CC<H78I!7HHI:D!C?J9BLQ?@:=D!C?E?;7:=!7HP:J87:<=:=D!R?JJ7H?87BHD!IL7@IHI87N<:!
!
S<ME7J?87BH=!;F7H8I9T8A!
− ;720%!LB!#*!1,:?!MB1'+0+1!1,C0(1'$!420%#$!N"O!()*&G0'#!2#$4&'$#$!*92&-69!1!L#(*0'HPQO17HPH%#+01*#+!
41*9R1)S:!T!;%%-'&,?!+&0U!PV:WVWXQY0%%-'&,:PZVZ[PP:!ZVP\!
− T:!],#0''0Y#'9-0$!#*!1,:?!MDBE!0'+-(#$!I^LZH+#4#'+#'*!'&'$4#(070(!42&*#(*0&'!72&%!2#0'7#(*0&'!801!
#406#'#*0(!2#42&621%%0'6!&7!%&'&()*#$S?!_IJ`?!+&0!UPV:PVa\Q4'1$:PZVZbaVPVX?!ZVPZ!
− ":!B9#'!#*!1,:?!MN21'$(204*0&'1,!+08#2$0*)!+-20'6!,0'#16#!(&%%0*%#'*!&7!9-%1'!C,&&+!
42&6#'0*&2$?S!`(0#'(#?!+&0U!PV:PPZ[Q$(0#'(#:PZcPV\\?!ZVPW:!
− `:!B9#'6!#*!1,:?!M%N^OH!1'+!d;eHP1f%#+01*#+!1#2&C0(!6,)(&,)$0$!1$!%#*1C&,0(!C1$0$!7&2!*210'#+!
0%%-'0*)?S!`(0#'(#?!+&0U!PV:PPZ[Q$(0#'(#:PZcV[bW?!ZVPW:!
− `:!`1##+!#*!1,:?!Mg406#'#*0(!42&621%%0'6!&7!%&'&()*#H*&H%1(2&4916#!+077#2#'*01*0&'!1'+!*210'#+!
0''1*#!0%%-'0*)?S!Science?!+&0U!PV:PPZ[Q$(0#'(#:PZcPVb[?!ZVPW:!
!
!