Translation Series No.1849

•
FISHERIES
RESEARCH
BOARD
OF CANADA
Translation
Series No. 1849
p ages
tYPescrii
,
t •
•
,,t
H5
Moulting hormones in the oenocytes of the meal beetle
•
y F. Romer
.
Original
title:
:Rautungshormone
in den Oenocyten des Mehlkafers
From:
Die -NaturwiSsenschaften
(The
Natural Sciences), 58(6): .
1
324-335, 1971
Translated by
. the
Translation
Bureau(
MV)
Foreign
Languages Division
.:Depa•ment
of the Secretary of State
of Canada-
Fisheries Research Board of
Canada
Halifax Laboratory
Halifax, N.
S.
1971.

/Re /ell,'
CANADA
•
TRANSLATED FROM - TRADUCTION DE
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EN
German
English
324
-3 25 •
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Berlin-Heidelberg-New York •
58 6
1971
5
Fisheries
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AUTHOR -
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ROMER
TITLE IN ENGLISH -
TITRE ANGLAIS_
• Moulting hormones
in theoenocytes of
the-meal beetle
.
Title
in
foreign
laaguago :(transliterate fareign-eharaetera)
Hâutungsnormone
in den
Oenotyten des Mehlkafers
.
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.The Natural Sciences
TRANSLATION
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0717
REQUEeTING DEPARTMENT
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ftEQUESTING
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• •
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Die
Maturwissehschaften, Volume 58, No.6, pp.324-325, 1971
Moulting hormones in
the'-oenocvtes
of the meal beetle
.324
•
By
•
F .
ROMER
•
Institut • fUr
. Allgemeine Zoologie
der. Univer-
'
sitU
Mainz * .
According to studïés
in the field of developmental physiolOgy,
the mbulting hormones.of
insects (ecdysones)
are
secreted by the
prothoracic glands [1,3].
Hitherto,
the isolation and structural '
identification of these hormones
he
been carried out on the
basis
of extracts from entire insects. Extraction
tests
with isolated
glands have confirmed this view
r7]
•
It
is also known, however, that
in
some beetles
and
butterflies the prothoracic glands
degenerate
shortly after pupation.
In spite
of a
very rapid degradation
system, the ecdysone content in
advanced pupal stages
is relatively
•
Institute -
forrGeneral
Zoology, Mainz Ilhiversity-
.-k
UNEDITED TRANSLATION
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seulement

2
high. This fact
and the nmults
of recent ligating experiments with
butterflies [9] suggest that there exists yet another
production
site of moulting
hormones.
Electron microscopic studies r6,21 have
shown that this
site might be
the oenocytes.
These latter, like
vertebrate steroid hormone-secreting cells, :ire characterized by
large amounts of agranular, tubular endoplasmic reticulum.
To test
this hypothesis,
large numbers of oenocytes
must
be isolated and worked
up.
Only
the Coleoptera and Lepidoptera
have
the anatomical conditions renuired for such
a procedure, and
Tenebrio molitor
L. (Tenebrionidae, Coleoptera) 'was chosen for
the
purpose.
The ecdysone titre
is particularly high sl-iortly before
pupation and in the
second half of the
pupal phase, and as a
result
it can be assumed that at those times
the activity
of the
hormone-
secreting cells has been intensified.
The oenocytes of 15 g prepupae were isolated
and stored
in a
deep-freeze compartment because
the preparation
of the insects
required
more than one week.
Concurrently, the prothoracic glands
and an equivalent amount of fat bodies
were isolated and,af ter
working up, subjected to
the Calliphora test r4]• So far, the
eXperiments have been-repeated four
times, with 'a pupation rate
'between .10
and 45% per
gram
of
starting material; 3
values lay

between 30
and
35%. The moulting hormone content. of
the prothoracic
glands was lower, on the average slighty more than half that
in
•
the oenocytes.
325
In the early pupal stages the
7,rothoracic
glands degenerate
ri
However,
tests the duration of which exceeded the
pupal stadium
(5 days at
32 °
C) have shown that the ecdysone
in the
4-day-old pupa
rises rapidly
and
amounts presumably to
7 CU/g
* , The activity found
in ,11e) oenocytes
was such that pupation took place in 40c ..,
or the
insects. This • corresponds to a
concentration of 2/3 CU/g in
the
oenocytes
of 4-day-old pupae. if the dry
substances of the extracted
tissues are related with each other,
the ratio of oenocytes
to
residual animals is approx.
1:40.
The
extracts from
corresnording
amount
s . of fat body
yielded lower concentrations. If these
value
e
.
are
extrapolated.
to - the total body•weight,
one finds that
in the
pupal
oènocytes the hormone content increases by
a factor
of 4,
while.in, the prepupa it rises by
a factor
of at least 10 since
• the tote ecdySone content is lower.
In addition
to and concurrent with
the
extraction experiments,
homogenates of isolated
prepupal oenocytes were injected into
(1) Tenebrio larvae which were undergoing
an
interval between
tle10
successive moults and had not started the next moulting,
and
(2)
Translator's note: The original German albreviation
"CE" stands
for "C....Einheit", leich translates as "C
Unit"; however, it
could not
- be established which unit
was actually referred
to.