O13 B. COSTANZA (1), A. BLOMME (1), E. MUTIJIMA (2), P. DELVENNE (3), O. DETRY (4), V. CASTRONOVO (1), A. TURTOI (1) / [1] University of Liege, Liège, Belgium,

O13
EXPEL:ANovelNonDestructiveMethodforMiningSolubleTumorBiomarkers
B.COSTANZA(1),A.BLOMME(1),E.MUTIJIMA(2),P.DELVENNE(3),O.DETRY
(4),V.CASTRONOVO(1),A.TURTOI(1)/[1]UniversityofLiege,Liège,Belgium,
MetastasisResearchLaboratory,GigaCancer,UniversityofLiege,Belgium,[2]University
HospitalLiege,Belgium,Liege,Belgium,DepartmentofPathology,,[3]UniversityHospital
Liege,Belgium,,Belgium,DepartmentofPathology,[4]UniversityofLiege,Liège,
Belgium,LaboratoryofMassSpectrometry,Dept.ofChemistry
Introduction:Secretedcancerproteinsareimportantmodulatorsoftumorgrowthand
progression.Thisproteingrouphasalwaysbeenconsideredamajorsourceofpotential
biomarkers.Unfortunatelyidentificationofnovelandclinicallyusefulsecretedtumorspecific
proteinsisdifficultsincetheirconcentrationinserumorurineisverylow.Alternativelyto
this,miningtissueproximalfluidhasemergedasapowerfulapproachtoidentifypotential
candidatebiomarkers.However,currentmethodsrelayonexvivoculturethattakes
considerabletimeandexposestissuebiopsiestouncontrolleddegradationthrough
endogenousproteases.
Aim:Aimoftheprojectisthediscoveryofnewsolublebiomarkersforearlydetection,
diagnosisorprognosisofhumancancerdisease.
Methods:Inthepresentworkwehavedevelopedanovelandefficientmethodforthe
collectionandanalysisoftumorsecretome.Theapproach,whichwetermedExpel,extracts
solubletumorbiomarkerswithinfewminutesandwithoutalteringthetissuemorphology.For
thispurposeasmalltissuebiopsyisincubatedinaslightlyhypertonicextractionbufferwhile
subjectedtoalternatingpressure.Uponextractionthetissueisfixedinformalinandcanbe
usedforhistologicalanalysis.Thesolubleextractisfurtherpreparedforproteomicanalysis
usingbottomupmassspectrometryapproach.
Results:Inaproofofconceptstudywehaveextractedandanalysedsolublebiomarkersfrom
humancolorectalcarcinomalivermetastasesaswellasprimarycolorectaltumors.Inan
extensivetissuevalidationstudyweconfirmthatExpelproceduredoesnotaltertissue
morphologyorsubsequentmolecularpathologytests.Thecomparisonofproteinsidentifiedin
tumorlesionswiththosefoundinadjacentnormaltissuesrevealedagroupofdifferentially
expressedsolubleproteins.Theirpotentialusefulnessasdiagnosticorpredictivemarkersis
currentlybeingexplored
Conclusions:TheExpelprotocolprovidesclinicianswithanewtoolenablingthemto
nondestructivelydiscovernewbiomarkersandpreserveprecioustissues(likecolonpolyps)
forpathologyevaluation
O14
Metabolomic,ProteomicandPreclinicalImagingofPatientDerivedTumorXenografts
forImprovingTreatmentofLiverMetastasesPatients
A.PÉREZPALACIOS(1),A.BLOMME(2),S.BOUTRY(3),G.DOUMONT(3),F.
SHERER(3),G.VANSIMAEYS(3),D.DEBOIS(4),G.JERUSALEM(5),J.
COLLIGNON(6),P.DELVENNE(7),O.DETRY(8),E.DEPAUW(4),R.MULLER(9),
S.GOLDMAN(10),V.CASTRONOVO(2),A.TURTOI(2)/[1]UniversityofLiege,
Liège,Belgium,MetatasisResearchLaboratory,[2]UniversityofLiege,Liège,Belgium,
MetastasisResearchLaboratory,[3]IRSPG,Gosselies,Belgium,CenterforMicroscopyand
MolecularImaging,[4]UniversityofLiege,Liège,Belgium,LaboratoryforMass
Spectrometry,[5]CHULiege,Liège,Belgium,DepartmentofMedicalOncology,,[6]CHU
Liege,Liège,Belgium,DepartmentofMedicalOncology,[7]UniversityofLiege,Liège,