O13 B. COSTANZA (1), A. BLOMME (1), E. MUTIJIMA (2), P. DELVENNE (3), O. DETRY (4), V. CASTRONOVO (1), A. TURTOI (1) / [1] University of Liege, Liège, Belgium,

O13
EXPEL:ANovelNonDestructiveMethodforMiningSolubleTumorBiomarkers
B.COSTANZA(1),A.BLOMME(1),E.MUTIJIMA(2),P.DELVENNE(3),O.DETRY
(4),V.CASTRONOVO(1),A.TURTOI(1)/[1]UniversityofLiege,Liège,Belgium,
MetastasisResearchLaboratory,GigaCancer,UniversityofLiege,Belgium,[2]University
HospitalLiege,Belgium,Liege,Belgium,DepartmentofPathology,,[3]UniversityHospital
Liege,Belgium,,Belgium,DepartmentofPathology,[4]UniversityofLiege,Liège,
Belgium,LaboratoryofMassSpectrometry,Dept.ofChemistry
Introduction:Secretedcancerproteinsareimportantmodulatorsoftumorgrowthand
progression.Thisproteingrouphasalwaysbeenconsideredamajorsourceofpotential
biomarkers.Unfortunatelyidentificationofnovelandclinicallyusefulsecretedtumorspecific
proteinsisdifficultsincetheirconcentrationinserumorurineisverylow.Alternativelyto
this,miningtissueproximalfluidhasemergedasapowerfulapproachtoidentifypotential
candidatebiomarkers.However,currentmethodsrelayonexvivoculturethattakes
considerabletimeandexposestissuebiopsiestouncontrolleddegradationthrough
endogenousproteases.
Aim:Aimoftheprojectisthediscoveryofnewsolublebiomarkersforearlydetection,
diagnosisorprognosisofhumancancerdisease.
Methods:Inthepresentworkwehavedevelopedanovelandefficientmethodforthe
collectionandanalysisoftumorsecretome.Theapproach,whichwetermedExpel,extracts
solubletumorbiomarkerswithinfewminutesandwithoutalteringthetissuemorphology.For
thispurposeasmalltissuebiopsyisincubatedinaslightlyhypertonicextractionbufferwhile
subjectedtoalternatingpressure.Uponextractionthetissueisfixedinformalinandcanbe
usedforhistologicalanalysis.Thesolubleextractisfurtherpreparedforproteomicanalysis
usingbottomupmassspectrometryapproach.
Results:Inaproofofconceptstudywehaveextractedandanalysedsolublebiomarkersfrom
humancolorectalcarcinomalivermetastasesaswellasprimarycolorectaltumors.Inan
extensivetissuevalidationstudyweconfirmthatExpelproceduredoesnotaltertissue
morphologyorsubsequentmolecularpathologytests.Thecomparisonofproteinsidentifiedin
tumorlesionswiththosefoundinadjacentnormaltissuesrevealedagroupofdifferentially
expressedsolubleproteins.Theirpotentialusefulnessasdiagnosticorpredictivemarkersis
currentlybeingexplored
Conclusions:TheExpelprotocolprovidesclinicianswithanewtoolenablingthemto
nondestructivelydiscovernewbiomarkersandpreserveprecioustissues(likecolonpolyps)
forpathologyevaluation
O14
Metabolomic,ProteomicandPreclinicalImagingofPatientDerivedTumorXenografts
forImprovingTreatmentofLiverMetastasesPatients
A.PÉREZPALACIOS(1),A.BLOMME(2),S.BOUTRY(3),G.DOUMONT(3),F.
SHERER(3),G.VANSIMAEYS(3),D.DEBOIS(4),G.JERUSALEM(5),J.
COLLIGNON(6),P.DELVENNE(7),O.DETRY(8),E.DEPAUW(4),R.MULLER(9),
S.GOLDMAN(10),V.CASTRONOVO(2),A.TURTOI(2)/[1]UniversityofLiege,
Liège,Belgium,MetatasisResearchLaboratory,[2]UniversityofLiege,Liège,Belgium,
MetastasisResearchLaboratory,[3]IRSPG,Gosselies,Belgium,CenterforMicroscopyand
MolecularImaging,[4]UniversityofLiege,Liège,Belgium,LaboratoryforMass
Spectrometry,[5]CHULiege,Liège,Belgium,DepartmentofMedicalOncology,,[6]CHU
Liege,Liège,Belgium,DepartmentofMedicalOncology,[7]UniversityofLiege,Liège,

Belgium,ExperimentalPathologyLaboratory,[8]UniversityofLiege,Liège,Belgium,
DepartmentofAbdominalSurgery,[9]IRSPG,Gosselies,Belgium,CenterforMicroscopy
andMolecularImaging(CMMI),[10]UniversityofLiege,Liège,Belgium,Centerfor
MicroscopyandMolecularImaging(CMMI),
Introduction:Successfultranslationalresearchreliesonanimaltumormodelsthatcan
veritablyrecapitulatethehumansituation.Mostisdonetodayusingmousexenografts,which
arebasedonculturedcancercells.However,thesecelllineshaveconsiderablydivergedfrom
theiroriginaltumors,criticallyloosingthegeneticandproteomicdiversitytypicallyfoundin
tumors.Thisdrawbackisattheoriginoftherecentsurgeofinterestinpatientderivedtumor
explants(PDTX),whichconsistsingraftinghumantumordirectlyinimmunocompromised
mice.
Aim:AlthoughthePDTXmodelispromising,littleisknownonhowwellarethefunctional
proteomicandmetabolomicaspectsconservedfromtheoriginalpatienttothemodel.Our
studyaimstobroadenthisknowledge.Afurtherquestionconcernstheclinicalutilityofthis
modelandtowhatextentcanfunctionaldataobtainedonPDTXhelpimprovetreatmentfor
cancerpatients.
Methods:InthecurrentstudywehavesuccessfullyestablishedPDTXfrom4individual
colorectalcarcinomaand4livermetastasespatients.Wehaveusedadvancedpreclinical
imagingbasedonPET/CT(18FDG)and9TMRItofunctionallymonitortumorbehaviorin
miceandcomparethisinformationtodataobtainedinthepatient.Allxenograftswere
evaluatedusingPERCISTcriteriaandcomparedtotheoriginalpatientdata.Wefurther
employmatrixassistedlaserdesorption/ionization(MALDI)imagingtechniquetoshowfor
thefirsttimeacomparativemetabolomicprofileofthepatienttumorandseveralgenerations
ofPDTX.
Results:OwingtoavalidatedsetofhistologicalmarkersweconfirmthatthePDTXmodels
haveconservedhistologicalfeatureswiththeoriginaltumor.Withthepreclinicalimagingand
theMALDIimagingtechniquewewereabletodeterminethemetabolicsignatureofthe
cancercellsandthestromarespectively,andwecouldassesstheimpactof
generationdependentevolutionofthePDTX.
Conclusions:Ourstudycontributestoabetterunderstandingofthebehaviorofthetumorand
itsevolutioninamodelthatcanbeusefulforbiomarkerdiscovery,outcomepredictionor
treatmentdesign.
Radiology,PathologyandNuclearMedicine
P01
Doesintraoperativepancreatoscopyaffectsurgicalmanagementofintraductalpapillary
mucinoustumorofthepancreas?
J.NAVEZ(1),J.GIGOT(2),C.HUBERT(2),P.DEPREZ(3),C.SEMPOUX(4),N.
JABBOUR(2)/[1]CliniquesUniversitairesStLuc,CityofBrussels,Belgium,Chirurgieet
transplantationabdominale,[2]CliniquesUniversitairesStLuc,CityofBrussels,Belgium,
ChirurgieetTransplantationAbdominale,[3]CliniquesUniversitairesStLuc,Cityof
Brussels,Belgium,Gastroentérologie,[4]CliniquesUniversitairesStLuc,CityofBrussels,
Belgium,Anatomiepathologique
Introduction:Becauseofthepotentialmalignantgrowthofintraductalpapillarymucinous
tumorofthepancreas(IPMTP),precisediagnosisofmalignantlesionsisessentialfor
completesurgicalresection.