Links between the ASCUS and the HPV in the 20-30 year-old women and 40-50 year Vanessa Roch Laboratory ADMED Pathology Neuchâtel, Switzerland Mentor: Patricia Besson Vuithier Introduction ASCUS (Atypical Squamous Cells of Undetermined Significance) is part of Bethesda 2001 system. These are squamous cells, in the cervix, with an enlarged nucleus, slightly irregular with sometimes a discret peri nuclear halo. (As you can see on the left hand of this picture). Its origin, as its name indicates, is not determined. It can result as well from a simple inflammatory reaction, like a sign of CIN (Cervix Intraepithelial Neoplasia) or more rarely a carcinoma and be associated with a HPV infection. HPV (human papillomavirus) is a sexually transmitted virus and is the main cause of cervix cancer. Aim Material and Methods My work consists in comparing the category ASCUS in search of HPV into two categories of women, the 20-30 year-old women and the 40-50 year-old women in order to see with which degree the ASCUS and the HPV are connected. The study was made over the year 20102011. The purpose of my work is also to demonstrate that this category has its importance in the Bethesda system. Classification of the results of HPV on the samples ASCUS of 185 women between 20-30 years and 199 women between 40-50 years of 2010-2011. The HPV results were classified by category of age as well as if they are positive or negative. Classification of the positive HPV in high HPV risks (HR), low risk (LR) or multiple infection high risk and low risk (HR/LR) was carried out. Search for biopsy associated with ASCUS. Results and interpretations Women between 20-30 years 2010-2011 HPV HR HPR LR HPR HR/LR 25% 47% 53% HPV + 16% 59% HPV - Women between 40-50 years 2010-2011 24% 76% HPV HR HPV + HPV - HPV LR HPV HR/LR 28% 17% 55% Discussion and Conclusion For the 20-30 year-old women, the diagnosis of ASCUS is important because it allows not to miss many high risk HPV. Indeed, in classifying these women as being negative, 82 women at risk of developing a cancer would have had no adequate follow-up. (That’s mean 50%). On the other hand, for the 40-50-year-old women, a diagnosis of ASCUS is very often associated with negative HPV. 75% of these women are thus followed and present no risk of developing a cancer.