Souches de parasites et de mollusques.................................................................. 55
Transformation in-vitro des miracidia en SpI ....................................................... 56
Récupération des Sporocystes secondaires et des échantillons contrôles ............ 56
Extraction protéique native des miracidia, Sp1 et SpII ........................................ 57
Effet dose ............................................................................................................... 57
Vaccination ........................................................................................................... 57
Analyses statistiques ............................................................................................. 58
RESULTATS ............................................................................................................................ 58
Effet dose ............................................................................................................... 58
Vaccination ........................................................................................................... 59
DISCUSSION ........................................................................................................................... 59
CHAPITRE TROIS : ............................................................................................................. 63
MECANISMES MOLECULAIRES DU PRIMING IMMUNITAIRE CHEZ
BIOMPHALARIA GLABRATA ............................................................................................. 63
A GLOBAL COMPARATIVE PROTEOMIC APPROACH TO INVESTIGATE THE FIRST HUMORAL
FACTORS RESPONSIBLE FOR IMMUNE PRIMING IN THE LOPHOTROCHOZOAN SNAIL
BIOMPHALARIA GLABRATA. ...................................................................................................... 64
ABSTRACT ......................................................................................................................... 68
1. INTRODUCTION ............................................................................................................ 70
2. MATERIAL AND METHODS ........................................................................................ 73
2.1. Ethic statements ............................................................................................. 73
2.2. Snail and parasite strains .............................................................................. 74
2.3. Histological procedures ................................................................................. 75
2.4. Plasma transfer .............................................................................................. 75
2.5. Proteomics ..................................................................................................... 76
2.6. Quantitative Real time PCR (qRT-PCR) ........................................................ 79
2.7. Statistical analysis .......................................................................................... 80
3. RESULTS ......................................................................................................................... 81
3.1. Histology ........................................................................................................ 81
3.2. Plasma transfer .............................................................................................. 81
3.3. Global comparative proteomic approach of primed snail plasma ................ 82
3.4. Quantitative Real Time PCR (qRT-PCR) ....................................................... 86
DISCUSSION ....................................................................................................................... 88
REFERENCES ..................................................................................................................... 97
CHAPITRE QUATRE : ...................................................................................................... 117