IBRB3004 Diagnostic de l`IBR

!
Page 1 sur 2!
05/11/14
IBRB3004'
Diagnostic'de'l’I.B.R'
!
!
• Recherche&sérologique&ou&recherche&d’anticorps&(ELISA)&
!
 Principe':''
'
"#!$%&'%$&'%!()*++,#*-.!/,*!-.+0*1#%!2),#%!*#3%&-*0#!$.&%#-%!0,!4#&*%##%5!
!
6%7!4#4(87%7!9%,:%#-!;-$%!$.4(*7.%7!%#!*#2*:*2,%(<!%#!+.(4#1%!2%!=!0,!2%!>?<!%#!30#&-*0#!2%!
()0@A%&-*3!$%&'%$&'.!%-!2%!(4!9$.:4(%#&%!BC!90,$&%#-41%!2%!907*-*37D!4--%#2,%5!
!
 Interprétation':'
'
E#!$.7,(-4-!907*-*3!%#!4#-*&0$97!FGH!7*1#*3*%!/,%!()4#*+4(!4!.-.!%#!&0#-4&-!4:%&!(%!:*$,7<!
/,)*(!%7-!9$0@4@(%+%#-!90$-%,$!%-!&0#-41*%,I!90,$!7%7!&0#1.#J$%7<!%-!/,)*(!(%!$%7-%$4!K!:*%!
+;+%!7)*(!$%2%:*%#-!7.$0#.14-*3!/,%(/,%7!+0*7!9(,7!-4$2!(0$7!2%!()%#-$.%!%#!(4-%#&%!2,!:*$,75!
!
E#!$.7,(-4-!#.14-*3!20*-!;-$%!*#-%$9$.-.!K!().&'%((%!2,!-$0,9%4,5!L*!(%!-$0,9%4,!%7-!*#2%+#%<!
0#!9%,-!:$4*7%+@(4@(%+%#-!9%#7%$!/,%!()4#*+4(!#.14-*3!#)4!A4+4*7!.-.!%#!&0#-4&-!4:%&!()FGH5!
M4$! &0#-$%! 24#7! ,#! -$0,9%4,! 907*-*3! %#! FGH<! 0#! 3%$4! 4--%#-*0#! K! #%! 947! 4&&0$2%$! -$09! 2%!
&0#3*4#&%! K! ,#! $.7,(-4-! 7.$0#.14-*3<! &4$! ()4#*+4(! 9%,-! ;-$%! ,#! 90$-%,$! (4-%#-! #)484#-! 947!
%#&0$%!2.:%(099.!2)4#-*&0$975!
!
 Résultats'apparemment'discordants':'
!
6%! 7,*:*!7.$0(01*/,%! 2%! ()FGH! %7-! 2*33*&*(%! &4$! +;+%! 7*! (%7! 4#*+4,I! 2%:*%##%#-!
30$-%+%#-! 7.$0907*-*37! /,%(/,%7! A0,$7! 49$J7! ()*#3%&-*0#<! (%! -4,I! 2)4#-*&0$97! @4*77%!
9$01$%77*:%+%#-!(0$7/,%!(%!:*$,7!%#-$%!%#!(4-%#&%5!
!
E#! 4#*+4(! 90$-%,$! 2%9,*7! 9(,7*%,$7! 4##.%7! 74#7! $.4&-*:4-*0#! 2,! :*$,7! 9%,-! 20#&! 4:0*$! ,#!
-4,I! 2)4#-*&0$97! *#3.$*%,$! 4,! 7%,*(! 2%! 7.$0907*-*:*-.<! %-! $%2%:%#*$! &0#-41*%,I! 74#7! 4,&,#!
78+9-N+%!:*7*@(%!K!()0&&47*0#!2),#!7-$%775!
!
!
O*#7*<!0#!9%,-!4:0*$!2%7!$.7,(-4-7!4994$%++%#-!2*7&0$24#-7!/,*!#%!70#-!947!2%7!%$$%,$7!P!
" $.7,(-4-!#.14-*3!9,*7!907*-*3!7,$!(%!+;+%!@0:*#!K!2%7!24-%7!9$0&'%7!P!(%!@0:*#!%7-!9%,-Q
;-$%!%#!&0,$7!2)4,1+%#-4-*0#!2%7!4#-*&0$97!7,*-%!K!,#%!*#3%&-*0#5!
!
" $.7,(-4-!907*-*3!9,*7!#.14-*3!7,$!(%!+;+%!@0:*#!K!2%7!24-%7!9$0&'%7!P!(%!@0:*#!4!9%,-Q
;-$%!.-.!*#3%&-.<!+4*7!(%!:*$,7!7)%7-!2*77*+,(.!24#7!(%7!14#1(*0#7!#%$:%,I!%-!()*++,#*-.!
%7-!%#!@4*77%5!F(!20*-!;-$%!&0#7*2.$.!R!K!$*7/,%!S!&4$!0#!#)4!4,&,#%!&%$-*-,2%!7,$!70#!
7-4-,-!$.%(5!M0,$!.:4(,%$!(%!#*:%4,!2%!$*7/,%<!0#!7)499,*%$4!7,$!()'*7-0$*/,%!%-!(%!7-4-,-!
*#2%+#%!0,!#0#!2,!-$0,9%4,5!
!
Rédacteur*:*Esthel*Thieri2Pigé*

!
Page 2 sur 2!
05/11/14
IBRB3004'
" $.7,(-4-7!2*7&0$24#-7!7,$!2%,I!4#4(87%7!$.4(*7.%7!7,$!(%!+;+%!-,@%!2%!74#1!4:%&!2%,I!
T*-7! 2*33.$%#-7!P! (%7! T*-7! 9%,:%#-! 9$.7%#-%$! 2%! (.1J$%7! 2*33.$%#&%7! 2%! 7%#7*@*(*-.! %-!
()*++,#*-.!2,!@0:*#!%7-!9$0@4@(%+%#-!9$0&'%!2,!7%,*(!2%!907*-*:*-.5!
!
U4#7! -0,7! (%7! &47<! 0#! 20*-! -0,A0,$7! *#-%$9$.-%$! (%7! $.7,(-4-7! K! ().&'%((%! 2),#! -$0,9%4,5! E#!
4#*+4(!7.$0#.14-*3!*77,!2),#!.(%:41%!2.-%#4#-!2%7!7.$0907*-*37!#%!20*-!A4+4*7!;-$%!&0#7*2.$.!
&0++%!74*#!V!!
!
E#! 4#*+4(! 7.$0907*-*3<! +;+%! 7)*(! $%2%:*%#-! 7.$0#.14-*3<! 20*-! ;-$%! &0#7*2.$.! &0++%! R!K!
$*7/,%!S!4:%&!,#!#*:%4,!2%!$*7/,%!/,*!2.9%#2!2,!7-4-,-!2,!&'%9-%(!:*7QKQ:*7!2%!()FGH5!
!
!
• Recherche&directe&(PCR)&
!
W#! &47! 2%! 7,79*&*0#! &(*#*/,%! 7,$! 2%7! 78+9-N+%7! $%79*$4-0*$%7! 0,! 2%7! 4:0$-%+%#-7<! 0#!
$%&'%$&'%!()F5G5H!94$!MXH!P!
" %#! &47! 2%! 78+9-N+%7! $%79*$4-0*$%7!P! .&0,:*((0#! #474(! 0,! (*/,*2%! 2)479*$4-*0#! -$4#7Q
-$4&'.4(%!
" %#! &47! 2%! 78+9-N+%7! -0,&'4#-! (4! $%9$02,&-*0#!P! .&0,:*((0#! 9$.9,&*4(<! .&0,:*((0#!
:41*#4(<!9(4&%#-4!0,!4:0$-0#!
!
X%7!$%&'%$&'%7!2*$%&-%7!70#-!$4$%+%#-!,-*(*74@(%7!&4$!(4!1$4#2%!+4A0$*-.!2%7!@0:*#7!907*-*37!
#)%I9$*+%#-!947!(4!+4(42*%<!%-!#)%I&$J-%#-!(%!:*$,7!/,%!2%!+4#*J$%!*#-%$+*--%#-%!K!()0&&47*0#!
2),#!7-$%775!64!$%&'%$&'%!2*$%&-%!#%!20*-!;-$%!,-*(*7.%!/,%!90,$!&0#3*$+%$!(4!$%790#74@*(*-.!2%!
()FGH!34&%! K! 2%7!7*1#%7! &(*#*/,%7<! %((%!#%! 9%$+%-! 947! 2)%I&(,$%! ,#! 90$-41%!2)FGH! 34&%! K! ,#%!
7.$0(01*%!907*-*:%5!!
!
!