Thesis-C.-Montante

Telechargé par Kooly Fiko
!
!
Université!de!Mons!
Faculté!des!Sciences!
Laboratoire!de!Protéomique!et!de!Microbiologie!
!
Functional!Study!of!the!Plasmid9Encoded!CopB!
Protein!Involved!in!Copper!Resistance!in!
Cupriavidus*metallidurans!CH34!
!
Thèse!présentée!en!vue!de!l'obtention!du!Grade!Légal!de!Docteur!en!
Sciences!Chimiques!par!Claire!MONTANTE!
!
!
Thèse!soutenue!publiquement!le!26!septembre!2014!devant!le!jury!composé!de:!
Prof.!Ruddy!Wattiez,!UMons,!Belgium! ! ! ! Directeur!de!thèse!
Dr.!Igor!Eeckhaut,!UMons,!Belgium! ! ! ! Président!du!jury!
Dr.!David!Gillan,!UMons,!Belgium!! ! ! ! Secrétaire!
Dr.!Jacques!Covès,!IBS,!Grenoble,!France!! ! ! Rapporteur!externe!!
Prof.!Meta!J.!Kuehn,!Duke!University,!NC,!USA! ! ! Rapporteur!externe!
Prof.!Max!Mergeay,!ULB,!SCK!•!CEN,!Belgium! ! ! Rapporteur!externe!
! !
!
!
To!my!friends!and!family,!with!all!my!love.!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
! !
!"
"
Abstract(
#$%%&'(" )*+,*" +-" %'&./0&12" +1" 2*&" &1.+'$13&12("+-" /1" &--&12+/0" 2'/,&" &0&3&12" '&45+'&6" 78" /00"
,&0050/'" $'9/1+-3-:" ;02*$59*" 2*+-"3&2/0" <50<+00-" 3/18" 7+$0$9+,/0" <51,2+$1-(" ,$%%&'"+-" /0-$" &=2'&3&08"
2$=+," 65&" 2$" +2-" /7+0+28" 2$" ,8,0&" 7&2)&&1" -2/70&" $=+6+>&6" #5?!!@" /16" 51-2/70&" '&65,&6" #5?!@" -2/2&-("
)*+,*" ,/1" 0&/6" 2$" ,&0050/'" 6/3/9&:" A*&" 65/0" '$0&" $<" ,$%%&'" ,$3%&0-" 3+,'$$'9/1+-3-(" -5,*" /-"
7/,2&'+/(" 2$" 6&.&0$%" '&-+-2/1,&" 3&,*/1+-3-" 2$" 2+9*208" ,$12'$0" ,$%%&'"*$3&$-2/-+-:"Cupriavidus*
metallidurans"-2'/+1" #BCD" +-" /" EF%'$2&$7/,2&'+53" /70&" 2$" '&-+-2" /16" 2$" -5'.+.&" +1" 2*&" %'&-&1,&" $<"
3/18" 3&2/0" +$1-" <$516" +1" /12*'$%$9&1+," 7+$2$%&-:" #$1-&45&1208(" C.* metallidurans"#BCD" +-" /1*
+3%$'2/12"3$6&0"<$'"-2568+19"'&-+-2/1,&"2$"153&'$5-"3&2/0-:"
#$%%&'" '&-+-2/1,&" +1" C.* metallidurans"#BCD" &--&12+/008" +1.$0.&-" 2*&" cop",05-2&'(" )*+,*" +-"
%'&-&12" $1" 2*&" %0/-3+6" %GHICJ(" copVTMKNSRABCDIJGFOLQHEW:" K0/-3+6F&1,$6&6" #$%" %'$2&+1-"
+1,056&"%'$2&+1-"+1.$0.&6"+1"%&'+%0/-3+,",$%%&'"6&2$=+<+,/2+$1("-5,*"/-"#$%LM;N#OP"%'$2&+1-"+1.$0.&6"
+1",82$%0/-3+,",$%%&'"6&2$=+<+,/2+$1(" -5,*"/-"#$%QP"/16"%'$2&+1-")*$-&"&=/,2"<51,2+$1-" '&3/+1" -2+00"
51R1$)1:" A*&" %0/-3+6F&1,$6&6" #$%N" %'$2&+1" */-" 9/+1&6"/22&12+$1"65&" 2$" +2-" 51+45&" SF2&'3+1/0"
&=2'&3+28(")*+,*"+-"'+,*"+1"3&2*+$1+1&"'&-+65&-:"A*&"<51,2+$1/0"-2568"$<"2*+-"%'$2&+1"+-"2*&"-57T&,2"$<"
2*+-"2*&-+-:" !12&'&-2+1908(" 2*&"%0/-3+6F&1,$6&6"#$%N"%'$2&+1"*/-"2)$"6+-2+1,2"-2'5,25'/0"6$3/+1-("/-"
-*$)1"&=%&'+3&12/008U"/1"51-2'5,25'&6"SF2&'3+1/0"&=2'&3+28(")*&'&+1"/"-&45&12+/0"3&2*+$1+1&F'+,*"
3$2+<"?MVWMOBLXMVWMOVWL@" +-"'&%&/2&6" 2&1"2+3&-(" /16"/"-2'5,25'/0"#F2&'3+1/0"6$3/+1(" )*+,*"
,$12/+1-"3/18"EF-2'/16-" /16" <&)" YF*&0+,&-:"L256+&-"$<"2*&-&"6$3/+1-"5-+19" -812*&2+,"%&%2+6&-"/16"
'&,$37+1/12" %'$2&+1-" 6&3$1-2'/2&6" /1" +12&'/,2+$1" 7&2)&&1" 2*&" -&45&12+/0" 3&2*+$1+1&F'+,*" 3$2+<"
/16"#5?!@"+$1-"/16("2$"/"0&--&'"&=2&12("#5?!!@"+$1-:"L57,&0050/'"<'/,2+$1/2+$1"/16"+3351$9$06"0/7&0+19"
$<" C.* metallidurans"#BCD" +1" 2*&" %'&-&1,&" $<" ,$%%&'" +16+,/2&6" 2*/2" 2*&" #$%N" %'$2&+1" )/-" $52&'F
3&37'/1&F/--$,+/2&6"/16" 3+9*2" 7&" <$06&6" +12$" /" EF7/''&0" ,$1<$'3/2+$1(" /-" -*$)1" 78" ,+',50/'"
6+,*'$+-3" /16" 2'/1-3&37'/1&" %'$2&+1" 2$%$0$98" %'&6+,2+$1:" #*&3+,/0" ,'$--F0+1R+19" &=%&'+3&12-"
-2'&192*&1&6"2*+-"-2568"/16"+6&12+<+&6"2*&"#$%;"%'$2&+1(")*+,*"+-"/"%52/2+.&"3502+,$%%&'"$=+6/-&("/-"
$1&"$<"2*&"#$%NF+12&'/,2+19"%/'21&'-"+1.$0.&6"+1"2*+-",$%%&'"'&-+-2/1,&"3&,*/1+-3:"
G$'&$.&'(" $5'" +1.&-2+9/2+$1-" /0-$" *+9*0+9*2&6" /" .&-+,50/2+$1" %*&1$3&1$1" 2*/2" */6" 1$2"
%'&.+$5-08"7&&1"6&-,'+7&6"+1"C.* metallidurans"#BCD"/16"/%%&/'-"2$"7&"-%&,+<+,"2$" ,$%%&'"&=%$-5'&"
/16"2*&"%'&-&1,&"$<"3&2/0F'&-+-2/1,&"9&1&-"*/'7$'&6"78"%0/-3+6-"?&:9:("%GHIZ["/16"%GHICJ@:"A*&"
*+9*" /7516/1,&" $<" ,$%%&'" +$1-" ,$3%0&=&6" 78" 2*&" %&'+%0/-3+," #$%" %'$2&+1-" +1-+6&" 2*&-&" $52&'"
3&37'/1&".&-+,0&-"-599&-2&6"2*/2"2*+-".&-+,50/2+$1"%'$65,2+$1"3/8"-&'.&"/-"/"1&)",$%%&'"'&-+-2/1,&"
3&,*/1+-3"+1"C.* metallidurans"#BCD:"A*+-"-2568"%/.&-" 2*&" )/8" 2$)/'6-" /" 7&22&'"516&'-2/16+19" $<"
2*&"</2&"$<",$%%&'"+1"2*+-"3+,'$$'9/1+-3:"
1 / 223 100%

Thesis-C.-Montante

Telechargé par Kooly Fiko
La catégorie de ce document est-elle correcte?
Merci pour votre participation!

Faire une suggestion

Avez-vous trouvé des erreurs dans linterface ou les textes ? Ou savez-vous comment améliorer linterface utilisateur de StudyLib ? Nhésitez pas à envoyer vos suggestions. Cest très important pour nous !