Nom : Habert

Stage proposé par Géraldine DELBES
Urology research labs, McGill University
Royal Victoria Hospital, H6-48
687 Pine Avenue west
Montreal, Quebec, H3A 1A1
Canada
Téléphone : 001-514-934-1934 ext 35490 (Chan) or 34574 (Delbes)
Mail : [email protected] and [email protected]
Site internet :
Directeur du Laboratoire ou de l’Unité : Peter CHAN
Intitulé de l ‘équipe d’accueil : Département de recherche en Urologie
Prénom et NOM du Responsable de l’équipe : Peter Chan
Résumé du thème de recherche de l’équipe (une dizaine de lignes maximum)
Research Interests
 Genetics in male infertility: genotype versus fertility; elaborating technique to better
select for the good sperm in assisted reproduction techniques.
 Chemotherapy impact on male fertility: Long term follow up of cancer survivor
fertility
 Microsurgery technique development: Testicular sperm extraction by aspiration and by
insertion
 Chromatin quality of human spermatozoa: Assessing the chromatin structure, maturity
and DNA bereaks using a molecular complementary panel of assays (SCSA, TUNEL,
Comet, CMA3 assays)
 Multi-media application in medical education
Titre du projet de stage :
Towards fertility improvement in testicular cancer patients.
Prénom, NOM, téléphone et adresse e-mail du Responsable du stage:
Geraldine Delbes
001-514-934-1934 ext 34574 (Delbes)
[email protected]
Projet de stage: Testicular cancer
is among the most prevalent neoplastic condition in young
men. With the availability in recent years of advanced diagnostic techniques and more
effective combination of chemotherapy regimens, many men in the reproductive age range
survive these malignancies with good quality of life. We have recently shown that the
chromatin and DNA quality of the sperm samples from cancer patients both pre and postchemotherapy, is abnormal, potentially leading to abnormal progeny outcome. We propose to
test the hypothesis that in cancer patients, sperm sample quality can be improved by
eliminating immotile and apoptotic sperm using gradient density and magnetic bead sorting
selections. The unique insight derived from these studies should permit the establishment of
biomarkers to predict sperm quality recovery and new strategies to enrich high quality sperm
in infertile cancer survivors, ultimately leading to clinically relevant information that will
benefit young cancer patients
Techniques mises en œuvre par le stagiaire :
1- Analyse de la chromatine des spermatozoïdes : TUNEL, COMET, Acridine orange
(SCSA®) and chromomycine A3
2- Sédimentation par gradient de densité
3- MACS, FACS
4- Extraction d’ADN génomique et PCR
Publications du Responsable de stage au cours des 5 dernières années :
PUBLICATIONS DE RECHERCHE:
- A. Yanagiya, G. Delbès, B. Robaire, N. Sonenberg. Translational Control in Late Spermiogenesis by
the PolyA Binding Protein Partner, Paip2A. J Clin Invest. 2010 Sep 1;120(9):3389-400
- G. Delbès, F. Vaisheva, T. Luu, L. Marcon, B. F. Hales, B. Robaire. Reversibility of the Effects of
the Chemotherapeutic Regimen for Non-Hodgkin Lymphoma, Cyclophosphamide, Doxorubicin,
Vincristine, and Prednisone, on the Male Rat Reproductive System and Progeny Outcome. Reprod
Toxicol. 2010 Jun;29(3):332-8.
- G. Delbès, D. Chan, P. Pakarinen, B.F. Hales, J.M. Trasler, B. Robaire. Impact of the Chemotherapy
Cocktail Used to Treat Testicular Cancer on the Gene Expression Profile of Male Germ Cells. Biol
Reprod. 2009 Feb;80(2):320-7.
- G. Delbès, C. Duquenne, J. Szencker, J. Taccoen, R. Habert, C. Levacher. Developmental changes
in testicular sensitivity to estrogens throughout fetal and neonatal life. Toxicol Sci. 2007
Sep;99(1):234-43.
- F. Vaisheva, G. Delbès, B.F. Hales, B. Robaire. Effects of combinaition chemotherapy with
cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP) on the male rat reproductive
system and progeny outcome. J Androl. 2007 Jul-Aug;28(4):578-87.
- G. Delbès, B.F. Hales, B. Robaire. Effect of the chemotherapy cocktail used to treat testicular cancer
on sperm chromatin integrity. J Androl. 2007 Mar-Apr;28(2):241-9; discussion 250-1.
- G. Livera, G. Delbès, C. Pairault, V. Rouiller Fabre, R. Habert. Organotypic culture, a powerful
model for studying rat and mouse fetal testis development. Cell Tissue Res, 2006 324(3):507-21.
CHAPITRE ET REVUES:
- R.J. Sherins, G. Delbès. Is there a Decline in Sperm Counts in Men? In Handbook of Andrology, 2nd
Edn (ed B. Robaire and P. Chan) 2010.
- G. Delbès, B.F. Hales, B. Robaire. Toxicants and human sperm chromatin integrity. Mol Hum
Reprod. 2010 Jan;16(1):14-22.
- G. Delbès, C. Levacher, R. Habert. Estrogen effects on fetal and neonatal testicular development.
Reproduction. 2006 Oct;132(4):527-38.
- R. Habert, G. Delbès, C. Duquenne, G. Livera, C. Levacher. Effects of estrogens on the development of
the testis during fetal and neonatal life. Gynecol Obstet Fertil. 2006, Oct;34(10):970-977
Autres informations:
Etudiants actuellement en thèse ou en M2 dans l’équipe d’accueil. Emilie Troeung
Etudiants ayant préparé ou soutenu leur thèse ou leur M2 dans l’équipe d’accueil au cours des
six dernières années. Aucun
Cette proposition de stage s’adresse-t-elle spécifiquement à un étudiant scientifique, médecin
ou vétérinaire ou bien est-il ouvert à tous les profils ? Tous les profils
Ce sujet peut-il donner lieu à une thèse ? Oui