Acta Stereologica ISSN : 0351-580X

Acta Stereologica
ISSN : 0351-580X
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Accueil / Volume 15 (1996) / Number 2 - Applications of stereology in life scie...
/ Influence of fixation, embedding and section mounting on stereological estimates of cancer cell mean volume-weighted nuclear volume
Morten LADEKARL & Hans SVANHOLM
Influence of fixation, embedding and section mounting on stereological estimates of cancer
cell mean volume-weighted nuclear volume
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AS_15_n2_165_LAD.pdf (1,7M)
Abstract
Objective malignancy grading of tumours by stereological estimation of the mean volume-weighted volume of cancer cell nuclei (VV(nuc)) might be clinically useful. Prior to
introduction in routine pathology, however, the effect of variations in tissue processing should be investigated. In the current study, the effect on VV(nuc) was assessed in 27
breast cancers regarding variations in formalin fixation and embedding medium, whereas the influence of the mounting procedure was studied in 14 bladder tumours. For
comparison, changes in gross dimensions of tumour specimens due to embedding were assessed in tissue from 5 breast cancers. Estimates of VV(nuc) were stable regarding
the duration and acidity of formalin fixation, but VV(nuc) was, on average, 13% larger in methaciylate than in paraffin (2p=0.004). Mounting of paraffin sections directly from water
provided a mean VV(nuc) about 30% larger than standard mounting from xylene or mounting from ethanol (p</=0.02). The overall mean linear shrinkage of tissue bars in paraffin
was 5%, whereas, in methaciylate, tissue bars swelled by 2% (2p>/=0.10). The dynamic changes in the dimensions of tissue were, however, dramatic during paraffin embedding
(up to 15%), whereas methaciylate embedded tissue was much more stable (up to 5% change). Thus, for reproducible assessments of VV(nuc), tissue processing should be
standardized with respect to the type of embedding medium and the section mounting procedure. As paraffin embedding leads to great dynamic changes in tissue dimensions,
the process is, most likely, sensitive, and should be strictly controlled. Mounting of paraffin sections from ethanol could replace routine mounting from the potentially toxic xylene
without affecting estimates of VV(nuc).
Keywords : malignancy grade, nuclear size, quantitative histopathology, tissue processing
Pour citer cet article
Morten LADEKARL & Hans SVANHOLM, «Influence of fixation, embedding and section mounting on stereological estimates of cancer cell mean volume-weighted nuclear volume»,
Acta Stereologica [En ligne], Volume 15 (1996), Number 2 - Applications of stereology in life sciences - July 1996, 165-169 URL : http://popups.ulg.ac.be/0351-580X/index.php?
id=579.
A propos de : Morten Ladekarl
Stereological Research Laboratory, Bartholin Building, University of Aarhus, DK-8000, Aarhus C
A propos de : Hans Svanholm
Department of Pathology, Randers Centralsygehus, DK-8900 Randers, Denmark
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