Acta Stereologica ISSN : 0351-580X A c c u Pe ri lé r s e e v n u t aS t t i a o t ni s dt ei q lu a e s e Accueil / Volume 15 (1996) / Number 2 - Applications of stereology in life scie... / Influence of fixation, embedding and section mounting on stereological estimates of cancer cell mean volume-weighted nuclear volume Votre recherche ... C hercher Recherche avancé Plus de Statistiques Morten L ADEKARL & Hans SVANHOLM Influence of fixation, embedding and section mounting on stereological estimates of cancer cell mean volume-weighted nuclear volume ➔Article depuis le 05 décembre 2013 : Visualisation(s): 111 (4 ULg) Téléchargement(s): 13 (0 ULg) Acta Stereologica Proceedings ICSIA Volume 18 (1999) Volume 17 (1998) Volume 15 (1996) Volume 14 (1995) Volume 13 (1994) Document(s) associé(s) Volume 12 (1993) Annexes AS_15_n2_165_LAD.pdf Volume 11 (1992) Volume 10 (1991) (1,7M) Volume 9 (1990) Volume 6 (1987) Abstract Volume 4 (1985) Objective malignancy grading of tumours by stereological estimation of the mean volume-weighted volume of cancer cell nuclei (VV(nuc)) might be clinically useful. Prior to introduction in routine pathology, however, the effect of variations in tissue processing should be investigated. In the current study, the effect on VV(nuc) was assessed in 27 breast cancers regarding variations in formalin fixation and embedding medium, whereas the influence of the mounting procedure was studied in 14 bladder tumours. For comparison, changes in gross dimensions of tumour specimens due to embedding were assessed in tissue from 5 breast cancers. Estimates of VV(nuc) were stable regarding the duration and acidity of formalin fixation, but VV(nuc) was, on average, 13% larger in methaciylate than in paraffin (2p=0.004). Mounting of paraffin sections directly from water Volume 3 (1984) Volume 2 (1983) Volume 1 (1982) Index provided a mean VV(nuc) about 30% larger than standard mounting from xylene or mounting from ethanol (p</=0.02). The overall mean linear shrinkage of tissue bars in paraffin Auteurs was 5%, whereas, in methaciylate, tissue bars swelled by 2% (2p>/=0.10). The dynamic changes in the dimensions of tissue were, however, dramatic during paraffin embedding (up to 15%), whereas methaciylate embedded tissue was much more stable (up to 5% change). Thus, for reproducible assessments of VV(nuc), tissue processing should be Keyword standardized with respect to the type of embedding medium and the section mounting procedure. As paraffin embedding leads to great dynamic changes in tissue dimensions, the process is, most likely, sensitive, and should be strictly controlled. Mounting of paraffin sections from ethanol could replace routine mounting from the potentially toxic xylene without affecting estimates of VV(nuc). Keywords : malignancy grade, nuclear size, quantitative histopathology, tissue processing Pour citer cet article Morten LADEKARL & Hans SVANHOLM , «Influence of fixation, embedding and section mounting on stereological estimates of cancer cell mean volume-weighted nuclear volume», Acta Stereologica [En ligne], Volume 15 (1996), Number 2 - Applications of stereology in life sciences - July 1996, 165-169 URL : http://popups.ulg.ac.be/0351-580X/index.php? id=579. A propos de : Morten Ladekarl Stereological Research Laboratory, Bartholin Building, University of Aarhus, DK-8000, Aarhus C A propos de : Hans Svanholm Department of Pathology, Randers Centralsygehus, DK-8900 Randers, Denmark Article précédent Article suivant Le portail PoPuPS est une réalisation du Réseau des Bibliothèques de l'Université de Liège. propulsé par Lodel | accès réservé Flux RSS ➔ Plan du site