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L‘interaction non-hôte Persil-Phytophthora sojae, un modèle pour étudier la réponse immunitaire innée chez les plantes Institut of plant biochemistry Pep-13, a minimal elicitor motif within GP42 Elicitor-active oligopeptide Open reading frame C P C XH C XH N RACE clones cDNA clone VWNQPVRGFKVYE Elicitor activity polypeptides expressed from subclones TS TSGPTS + + + Institute of Plant Biochemistry Catalytic mode of action of transglutaminases NH3 O Protein Gln C NH2 SH + Protein Cys O Gln C S Cys Enzyme Enzyme SH Cys Enzyme O Proteine Gln C S + Cys R NH2 Proteine O Gln C NH R Enzyme R = Amines, Lysine Institut of plant biochemistry GP42 TGase homologs are highly conserved among the genus Phytophthora Institute of Plant Biochemistry Pep-13-containing GP42 TGase homologs are highly conserved among Phytophthora spp. Institute of Plant Biochemistry IPB Hydropathy plot of Phytophthora sojae transglutaminase Pep-13 is part of a surface-exposed loop structure Institute of Plant Biochemistry IPB TGase and elicitor activities of P. sojae wild-type and mutant proteins Pep-13 is functionally indispensable Institute of Plant Biochemistry IPB Pep-13 elicits defense reactions in potato with the same signal specificity as in parsley P234A W231A Institute of Plant Biochemistry