1-s2.0-S266592712200017X-mmc1

Supplementary Material
Digestibility of vitamin K (phylloquinone and menaquinones) in
food and supplements
Marie Bagge Jensena*, Anja Pia Biltoft-Jensenb, Jette Jakobsena
aResearch Group for Bioactives - Analysis and Application, National Food Institute, Technical University
of Denmark, Kemitorvet, 2800 Kgs. Lyngby, Denmark
bResearch Group for Nutrition, Sustainability and Health Promotion, National Food Institute, Technical
University of Denmark, Kemitorvet, 2800 Kgs. Lyngby, Denmark
Marie Bagge Jensen, [e-mail].
Table of Contents
SM 1…………………………………...…………………………………………………………..1
SM2………………………………………………………………………………………………..2

1
SM 1: Four fractional factorial (23-1) experiments performed in order to validate the INFOGEST
2.0 model for assessment of bioaccessibility of vitamin K vitamers. All samples were digested in
triplicates (n=3) and analysed for vitamin K vitamers using the LC-ESI-MS/MS method (Jensen
et al., 2021b).
Experiment 1
Result
Sample
1
Sample 2
Sample
3
Sample
4
Factor
0.8 g Raw spinach (A)/
0.8 g Frozen boiled spinach (a)
A
A
a
a
p-value <
0.05
0.2 g Oil (B)/
No oil (b)
B
b
B
b
p-value <
0.05
Bile (C)/
2 x bile (c)
C
c
c
C
p-value <
0.05
Bioaccessibility (%)
D
E
F
G
Experiment 2
Result
Sample
1
Sample 2
Sample
3
Sample
4
Factor
100 ng PK, MK-4, MK-7, MK-9 (A)/
200 ng PK, MK-4, MK-7, MK-9 (a)
A
A
a
a
p-value >
0.05
100 ng vitamin D3 (B)/
No vitamin D3 (b)
B
b
B
b
p-value >
0.05
Bile (C)/
2 x bile (c)
C
c
c
C
p-value >
0.05
Result
D
E
F
G
Experiment 3
Result
Sample
1
Sample 2
Sample
3
Sample
4
Factor
0.8 g standard meal (A)/
0.4 g standard meal (a)
A
A
a
a
p-value <
0.05
0.1 g raw spinach (B)/
0.2 g raw spinach (b)
B
b
B
b
p-value >
0.05
Bile (C)/
2 x bile (c)
C
c
c
C
p-value >
0.05
Result
D
E
F
G
Experiment 4
Result
Sample
1
Sample 2
Sample
3
Sample
4
Factor
0.8 g standard meal (A)/
0.6 g standard meal (a)
A
A
a
a
p-value <
0.05
500 ng PK, MK-4, MK-7, MK-9 (B)/
1000 ng PK, MK-4, MK-7, MK-9 (b)
B
b
B
b
p-value >
0.05
500 ng vitamin D3 (C)/
1000 ng vitamin D3 (c)
C
c
c
C
p-value >
0.05
Result
D
E
F
G

2
SM2: Chromatogram of vitamin K vitamers and internal standards in a natto sample. The sample
was analysed for PK, MK-4, MK-5, MK-6, MK-7, MK-9, MK-10, d7-PK, d7-MK-4, d7-MK-7 and
d7-MK-9. Only the highest peaks are indicated by arrows and labels.