micropropagation Cours + protoplast + ES + MICROGREFFAGE

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Techniques of Micropropagation
Chapter 18
Systems used to regenerate plantlets by
micropropagation
I.) Axillary shoot formation
Meristem tip culture
Results in plantlets free from viruses, fungi and bacteria
(esp. when coupled with heat treatment)
Important for many herbaceous crops (carnations, mums,
orchids, geraniums, banana, potato, sweetpotato)
With woody plants, meristems are often grafted
Axillary shoot culture
Reliably reproduces the genotype of the parent plant
(expands existing buds)
Carnation meristem
Nodal shoot production at cotyledonary stage
Systems used to regenerate plantlets by
micropropagation
Adventitious shoot formation
Initiated directly on the explant or indirectly from
callus
Results in high rates of multiplication
Results in increased aberrant (“off-type”) plants
Parts used:
Leaf pieces (ie: African violet)
Cotyledons (ie: conifers)
Immature inflorescence (ie: Hosta and daylily)
Bulb scales (ie: Easter lily, hyacinths, etc.)
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micropropagation Cours + protoplast + ES + MICROGREFFAGE

Telechargé par chokribayoudh
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