micropropagation Cours + protoplast + ES + MICROGREFFAGE
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Techniques of Micropropagation
Chapter 18
Systems used to regenerate plantlets by
micropropagation
•I.) Axillary shoot formation
–Meristem tip culture
•Results in plantlets free from viruses, fungi and bacteria
(esp. when coupled with heat treatment)
•Important for many herbaceous crops (carnations, mums,
orchids, geraniums, banana, potato, sweetpotato)
•With woody plants, meristems are often grafted
–Axillary shoot culture
•Reliably reproduces the genotype of the parent plant
(expands existing buds)
Carnation meristem
Nodal shoot production at cotyledonary stage
Systems used to regenerate plantlets by
micropropagation
•Adventitious shoot formation
–Initiated directly on the explant or indirectly from
callus
–Results in high rates of multiplication
–Results in increased aberrant (“off-type”) plants
–Parts used:
•Leaf pieces (ie: African violet)
•Cotyledons (ie: conifers)
•Immature inflorescence (ie: Hosta and daylily)
•Bulb scales (ie: Easter lily, hyacinths, etc.)
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