Laboratoire Plastes et Différenciation Cellulaire - UMR 5575
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Laboratoire Plastes et Différenciation Cellulaire - UMR 5575 Proposition d’accueil d’ATIP 2006 The CNRS UMR 5575 “Plastes et Différenciation Cellulaire” (http://www-pdc.ujf-grenoble.fr) wishes to host an ATIP team in 2006. The UMR is located on the campus of Université Joseph Fourier in Grenoble (French Alpes). It is composed of 22 permanent researchers and technicians, 14 post-doctoral fellows and PhD students, and 6 undergraduates. The main topics actually developed in the unit concern molecular mechanisms and molecular complexes involved in plastids and cell differentiation, in response to light and temperature stress and/or hormone signals. We use combinations of biochemistry, cell biology, trancriptomics and reverse and direct genetics. Besides access to the common facilities, a surface of at least 50 m2 will be allocated to the new team. Theme 1: Plastids Differentiation and Senescence (Steffen REINBOTHE) ([email protected]) We are interested in understanding how plants turn green after germination, how photosynthesis becomes established in angiosperm seedlings. We have characterized a new light harvesting complex (LHPP) located in the prolamelar body of chloroplasts. Another goal of our research is the study of the importation into the chloroplast of the POR enzymes in relation to chloroplast development. We recently found that PORA is imported via a new membrane protein complex, the protochorophyllide translocon (Ptc). Theme 2: Transcription and Division of Plastids (Silva LERBS-MACHE) ([email protected]) We are interested to study the processes implicated in transcription of the plastid genome and in division of the plastid. In particular, we are characterizing the RNA polymerases and transcription factors that function in the transcription of the plastid genome as well as the proteins that play major roles in plastid division. Theme 3: Gibberellins and Cell Differentiation (Michel HERZOG) ( [email protected] ) We are interested in gibberellin hormones (GA) signaling and their role in cell differentiation. We demonstrated links between GA signaling and molecular events that govern trichome (hair) initiation and trichome cell morphogenesis. Current research focuses on a new family of plant specific transcription factors, named GeBP, controled by BREVIPEDICELLUS, a KNOX homeobox regulator of organogenesis. BP downregulates GA biosynthesis in the vicinity of the shoot apical meristem and shows expression patterns comparable to GeBPs. Scientific environment of the UMR 5575 « Plaste et Différenciation Cellulaire » The UMR has developped fruitful scientific and/or technologic interactions with several labs or Institutes based on University Campus or on the more distant CEA campus - Laboratoire de Physiologie Cellulaire Végétale (UMR 5168 CNRS-INRA-CEA-UJF) - Laboratoire d’Ecologie Alpine (UMR 5553) - Centre d’Etude sur les Macromolécules Végétale (CERMAV, UPR 5301) - Unité Mixte de Service “Jardin Alpin Joseph Fourier” (UMS 2925 CNRS-UJF) (greenhouse facility) - Laboratoire de Bioénergétique Fondamentale et Appliquée (INSERM Technological competence of our UMR Expression of the plastidial genome - Subcellular splitting up – Plant and Plastids transformation. - In vitro reconstitution of nanomolecular complexes - Cell biology - Genomics and genetic engineering - Protein biochemistry and engineering – Cell and tissue imaging – immunodetection Available Laboratory equipments The UMR provides necessary equipments for molecular biology, protein and nucleic acids analysis, Cell biology and confocal imaging, Plants and tissue culture facilities Technological Platforms accessible or shared with neighbor laboratories Confocal microscopy LEICA FCS-SP2, 4 lasers (including UV) - Electron microscopy - DNA Sequencing - High throughput Genotyping - Mass spectrometer - New Greenhouse and plant culture facility (coming soon) Contact : Pr Michel Herzog Directeur, Tel: (33) 04 76 51 48 01, Fax: (33) 04 76 51 43 36 [email protected]; http://www-pdc.ujf-grenoble.fr Adresse postale : Université J. Fourier, CERMO, BP53, F-38041 Grenoble Cedex 9. Tél : (33) 04 76 51 48 01 Fax : (33) 04 76 51 43 36 Adresse géographique : 460 rue de la Piscine, Domaine universitaire – 38400 Saint Martin d’Hères - [email protected]
