Anti-5 methylcytosine monoclonal antibodies PUBLICATIONS 1985-1990 Adouard, V., Dante, R., Niveleau, A., Revet, B., Delain, E. and Ehrlich, M. (1985) The accessibility of 5-methylcytosine to specific antibodies in double-stranded DNA of Xanthomonas phage XP12 Europ. J. Biochem. 152 115-121 C. Reynaud, A. Niveleau 1(990) Methylated bases as tumor markers.: Detection and quantitation with a competitive enzyme-linked immunoassay. Anal. Letters 23, 31-45 C. Reynaud, C. Bruno, P. Boullanger, J. Grange, S. Barbesti and A. Niveleau. Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies Cancer Letters (1991) 61 255-262 1991-1995 R. Dante, A. Baldini, D.A. Miller, M. Rocchi, A. Niveleau and O.J. Miller. (1991) Methylation des régions 5' promotrices de l'ARN ribosomal humain dans des lignées lymphoblastoides et un hybride somatique humain-hamster. in Chromatine Aspects structuraux et fonctionnels. Editions du CNRS 297-305 C. Reynaud, C. Bruno, P. Estrabaud, S. Barbesti and A. Niveleau. (1992) Monitoring the excretion of modified nucleosides by cancer patients with a six-monoclonal antibody set in a solid-phase immunoassay. Amer. Assoc. Cancer Research. 33, 201.(Abstarct) R. Dante, A. Baldini, D.A. Miller, M. Rocchi and A. Niveleau. (1992) Methylation of the 5' flanking sequences of the ribosomal DNA in human cell lines and in a humanhamster hybrid cell line. J. Cell. Biochem. 50, 357-362. C. Reynaud, L. Frappart and A. Niveleau (1994) Immunochemical detection of modified nucleosides in DNA and RNA in cells grown in vitro and in breast tissues 85th Annual Meeting. Amer. Ass. Cancer Research 35, 15 (Abstract) Montpellier, C. Bourgeois, C., Kokalj-Vokac, N., Muleris,M. ,Niveleau, A. , Gibault, A., Malfoy, B. and Dutrillaux,B. (1994 Detection of methylcytosine rich heterochromatin on banded chromosomes . Application to cells with various status of DNA methylation. Cancer Genetics Cytogenetics. 78, 87-93 Barbin A., Montpellier,C., Kokalj-Vokac, N.,Gibaud,A., Niveleau, A., Dutrillaux, B., Malfoy, B. and Bourgeois C.A. (1994) In situ DNA methylation analysis of human tumor cells Mapping of methylcytosine-rich bands. Human Genetics, 94, 684-692 Niveleau, A., Drouet, C., Bruno, C. Reynaud, , C., Fares, F. (1994) Polymerase chain reaction products containing 5-methylcytidine. A microplate immunoquantification method. Nucleic Acids Res. 22, 5508-5509 Delain,E., Barbin-Arbogast,A., Bourgeois,C., Mathis,G., Moryd,C., Favard,C. and Niveleau,A.. (1995) The limits in electron microcopy of macromolecules interactions. The use of new labels based on lanthanide cryptates. An interdisciplinary approach.Journal of Trace and Microprobes Techniques, 13(3), 371-381 Miniou,P., Jeanpierre,M., Blanquet,V.,Sibella,V., Bonneau,D., Herbelin,C., Fischer,A., Niveleau,A. & Viegas-Péquignot,E. (1994) Abnormal methylation pattern in constitutive and facultative (X inactive chromosome) heterochromatin of ICF patients. Human Mol. Genetics 3, 2093-2102 Veilleux,C., Bernardino,J., Gibaud,A.,Niveleau,A., Malfoy,B., Dutrillaux,B. et Bourgeois,C. (1995) Alterations de la methylation dans les cellules tumorales: approche quantitative "in situ". Bull Cancer 82, 939-945 1996-2000 de Capoa, A., Menendez,F., Poggesi, I., Grapelli, C., Marotta, M.R., Di Leandro, M., Reynaud, C., Niveleau, A. (1996) Cytological evidence for 5-azacytidine-induced demethylation of the heterochromatic regions of human chromosomes Chromosome Research, 4,271-276. Bernardino,J., Lamoliatte,E., Lombard,M., Niveleau,A., Malfoy,B., Dutrillaux,B., Bourgeois,C.A. (1996) DNA methylation of the X chromosomes of the human female: an in situ semi-quantitative analysis. Chromosoma, 104: 528-535. Habib, M., Bourgeois, C.A., Truong,K., Bernardino, J., Dutrillaux, B., Souchier, C., Niveleau, A. (1996) DNA methylation and cancer: an in situ approach. Proceedings American Association against Cancer. 37, 535 (abstract) Sasco, A.J., Rey, F., Reynaud, C., Bobin, J.Y., Clave, M. , Niveleau, A. (1996) Breast cancer prognostic significance of some modified urinary nucleosides. Cancer Letters, 108, 157-162. Tweedie, S., Charlton, J., Clark, V., Bird, A. (1997). Methylation of genomes and genes at the invertebrate-vertebrate boundary. Molecular and cancer biology 17: 1469-1475. Bensaada, M., Kiefer, H., Tachdjian,G., Lapierre, J.-M., Cacheux, V., Niveleau, A., Métézeau, P. (1998) Altered paterns of DNA methylation on chromosomes from leukemia cell lines.Identification of 5methylcytosines by indirect immunodetection. Cancer Genet Cell Genet. 103: 101-109. Tebib, J.G., Reynaud, C., Cedoz, J.P., Letroublon, M.C., Niveleau, A. (1997). Relationship between urinary excretion of modified nucleosides and rheumatoid arthritis process. Br. J. Rheumatol. 36, 990-995. Kokalj-Vokac N., Zagorac, A., Pristovnik, M., Bourgeois, C.A., Dutrillaux, B. (1998) DNA methylation of the extraembryonic tissues: an in situ study on human metaphase chromosomes. Chromosome Res, 6: 161-166. de Capoa A, Di Leandro M,Grappelli C, Menendez F, Poggesi I, Giancotti P, Marotta MR, Spanò A, Rocchi M, Archidiacono N, Niveleau A (1998) Methylation status of individual interphase nuclei in human cultured cells: A semiquantitative computerised analysis. Cytometry, 31, 85-92. Habib, M., Fares, F., Drouet, E., Niveleau, A. (1998) DNA methyltransferase activity and DNA methylation levels: evaluation with a non-radioactive technique. Proceedings 89 th Annual Meeting AACR.39, 94 (Selected Abstract) Rougier, N., Bourch’is, D., Molina, D., Niveleau, A., Plachot, M., Paldi, A., Viegas-Pequignot, E. (1998) Chromosome methylation patterns during mammalian pre-implantation development. Genes and Dev. 14, 2108-2113. Benchaib, M., Ajina, M., Souchier, C., Niveleau, A., Lornage, J., Guerin, J.-F. (1998) A protocol to investigate DNA methylation staining by image analysis in human spermatozoa.. In Genetics of human male fertility. C Barratt, C. De Jonge, D. Mortimer, J. Parinaud. Eds. EDK 1997 222-231. de Capoa, A., Romana Febbo, F., Giovanelli, F., Niveleau, A., Zardo, G., Marenzi, S., Caiafa, P. (1999) Reduced levels of Poly(ADP)-ribosylation result in chromatin compaction and hypermethylation as shown by a cell-by-cell computer-assited quantitative analysis. FASEB. J. 13, 89-93. Habib, M., Fares, Bella, C., F., Bourgeois,C.A., Bernardino,J., Blazquez-Hernandez, F., de Capoa A, Niveleau, A. (1999) DNA global hypomethylation in EBV-transformed interphase nuclei. Exp. Cell Res. 249, 46-53 de Capoa, A., Grappelli, C., Febbo, F.R, Spano, A., Niveleau, A., Cafolla, A., Cordone, I., Foa, A. (1999) Methylation levels of normal and chronic lymphocytic leukemia B lymphocytes: computer-assisted quantitative analysis of anti-5-methylcytosine antibody binding to individual nuclei. Cytometry, 36, 157-159 Xu, G.-L., Bestor, T.H., Bourc’his, D., Hsieh, C.-L., Tommerup, N., Bugge, M., Hulten, M., Qu, X., Russo, J.J., Viegas-Pequignot, E. (1999) Chromosome instability and immunodeficiency syndrome caused by mutations in a DNA methyltransferase gene. Nature, 402: 187-191. Mayer, W., Niveleau, A., Walter, J., Fundele, R., Haaf, T. (2000) Active demethylation of the zygotic paternal genome. Nature 403: 501-502. Coffigny, H., Bourgeois, C., Ricoul, M., Bernardino, J., Vilain, A., Niveleau, A., Malfoy, B. Dutrillaux, B. (1999) Alterations of DNA methylation patterns in germ and Sertoli cells from developing mouse testis. Cytogenet. Cell. Genet. 87: 175-181. Stancheva, I. and Meehan, R.M. (2000) Transient depletion of xDnmt1 leads to premature gene activatioon in Xenopus embryos. Genes and Development, 14: 313-327. Bernardino J, Lombard M, Niveleau A, Dutrillaux B (2000). Common methylation characteristics of sex chromosomes in somatic and germ cells from mouse, lemur and human. Chromosome Res.;8(6):513-25. F.J. Hernandez-Blazquez, M. Habib, J.-M. Dumollard, C. Barthelemy, M. Benchaib , A. de Capoa, A. Niveleau. (2000) Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. Gut 47: 689-693. Vilain A, Bernardino J, Gerbault-Seureau M, Vogt N, Niveleau A, Lefrancois D, Malfoy B, Dutrillaux B. (2000) DNA methylation and chromosome instability in lymphoblastoid cell lines. Cytogenet Cell Genet.;90(1-2):93-101. Zagradisnik B, Kokalj-Vokac N. (2000) Hypomethylation of alphoid DNA and classical satellite DNA on chromosome 1, 9, 16 and Y in extraembryonic tissue. Pflugers Arch.;440(5 Suppl):R190-2. 2001Taddei, A., Maison, C., Roche, D., Almouzni, G. (2001) Reversible disruption of pericentric heterochromatin and centromere function by inhibiting deacetylases in mammalian cells. Nature Cell Biol, 3 :114-120 Piyathilake CJ, Johanning GL, Frost AR, Whiteside MA, Manne U, Grizzle WE, Heimburger DC, Niveleau A. (2000) Immunohistochemical evaluation of global DNA methylation: comparison with in vitro radiolabeled methyl incorporation assay. Biotech Histochem. 2000 Nov;75(6):251-8. F. Grützner, E. Zend-Ajusch, K. Stout, S. Munsche, A. Niveleau, I. Nanda, M. Schmid, and T. Haaf (2001) Chicken microchromosomes are hypermethylated and can be identified by specific painting probes Cytogenet. Cell Genet., 93:265-269. Piyathilake CJ, Frost AR, Bell WC, Oelschlager D, Weiss H, Johanning GL, Niveleau A, Heimburger DC, Grizzle WE. (2001) Altered global methylation of DNA: An epigenetic difference in susceptibility for lung cancer is associated with its progression. Human Pathol. 32(8):856-62 Bourch’is, D., Le Bourhis, D., Patin, D., Niveleau, A., Comizzoli, P., Renard, J.P., Viegas-Pequignot, E. (2001). Delayed and incomplete reprogramming of chromosome methylation patterns in bovine cloned embryos. Current biology, 11:1542-1546 Barton, S.C., Arney, K.L., Shi, W., Niveleau, A., Fundele, R., Surani, M.A., Haaf, T. (2001) Genome-wide methylation patterns in normal and uniparental early mouse embryos. Human Mol. Genetics. 10:2983-2987 Stancheva, I., El-Maarri, O., Walter, J., Niveleau,A. Meehan, R.R. (2002). DNA methylation at promoter regions regulate the timing of gene activation in Xaenopus laevis embryos. Dev. Biol. 243:155-165 COMMUNICATIONS A. Niveleau, C. Reynaud and J. Grange Urinary excretion of non protein tumor markers: monitoring by EIA. Journées immunologiques. Fondation franco-allemande. Paris 24-26 Novembre 1988 Niveleau, A. Bases puriques et pyrimidiques modifiées. 6° Symposium Biochimie Clinique des Cancers. Fondation Bergonié. 27-29 Avril 1989 Bordeaux. Dante, R., Baldini, A., Miller, D.A., Rocchi, M. and Niveleau, A. Methylation du promoteur du rDNA humain dans les lymphocytes humains et des hybrides somatiques. Forum des jeunes chercheurs. Juin 1990 Lyon. Reynaud, C., Bruno, C., Niveleau, A., Boullanger, P., et J Grange J. Dosage immunoenzymatique à l'aide d'anticorps monoclonaux de bases modifiées dans les liquides biologiques. Colloque régional INSERM. 17-19 Octobre 1990.Lyon. L. Frappart, C. Reynaud, C. Bruno and A. Niveleau Modified nucleosides in malignancy. Detection, visualization and quantification in human tissues and urine with a set of monoclonal antibodies. European Workshop on genetic alterations in human solid tumors. 9-11 Mai 1991 Montpellier C. Reynaud, C. Bruno and A. Niveleau Les nucléosides modifiés en cancérologie clinique Dosage de l'excrétion urinaire au moyen de six anticorps monoclonaux. Optimisation des essai. Etude statistique sur 200 cas. VII° Symposium Biochimie Clinique des Cancers Deauville 15-16 Mai 1991 L. Frappart, C. Pechoux, C. Reynaud et A. Niveleau Les nucléosides modifiés en cancérologie clinique. Etude immunocytochimique de l'accessibilité de la 5-methylcytidine et de la pseudo-uridine dans les lésions bénignes et malignes du sein. VII° Symposium Biochimie Clinique des Cancers. Deauville 15-16 Mai. S. Barbesti, A. Niveleau, C. Reynaud and P. Giordano. A new enzyme immunoassay for modified nucleosides. 25 Years of immunoenzymatic techniques International Congress Athens September 9-12.1991 C. Reynaud, P. Bruno, P. Boullanger, J. Grange, S. Barbesti and A. Niveleau Excretion of modified nucleosides by cancer patients: monitoring by EIA. Tumor markers: current applications and impact on Therapy. 8th International Conference of IATMO. Nice 16-19 Novembre 1991. S. Barbesti, A. Niveleau, C. Reynaud, C. Bruno, M. Danova and P. Giordano. Monoclonal antibodies for new tumor marker immunoassay tests. International Symposium "New generation of monoclonal antibodies in diagnosis and therapy" Gênes (Italie) 12-15 Avril 1992 C. Reynaud, P. Estrabaud , E. Archimbaud, A. Perret-Liaudet, P. Later,and A. Niveleau. Monitoring the excretion of modified nucleosides in haematological malignancies. Conference on Anticancer Research. Crête, Grèce. Octobre 1992 L. Frappart , C. Reynaud, C. Pechoux ,and A. NIVELEAU. Modified nucleosides in clinical oncology: Studies on the accessibility of 5-methylcytidine and pseudouridine-containing macromolecules to monoclonal antibodies in breast tumors. Fourth International Conference on Anticancer Resarch. Crête, Grèce. Octobre 1992 A. Niveleau. C. Reynaud and C. Bruno. Non-protein tumor markers:modified nucleosides. 17 th Congress of the European Society for Medical Oncology. Lyon. France. Novembre 1992. C. Reynaud, P. Estrabaud , E. Archimbaud, A. Perret-Liaudet, P. Later,and A. Niveleau. Monitoring the excretion of modified nucleosides in haematological malignancies. 17 th Congress of the European Society for Medical Oncology. Lyon. France. Novembre 1992. C. Reynaud, C. Bruno,.E. Archimbaud & A. Niveleau. Post-therapeutic and long term monitoring of Cancer patients: modified nucleosides excretion. 17 th Congress of the European Society for Medical Oncology. Lyon. France. Novembre 1992. J.G. Tebib, C. Reynaud, M. Letroublon, A. Niveleau and M. Bouvier Relationship between the urinary excretion of modified nucleosides and the rheumatoid arthritis process. American College of Rheumatology 57 th nnual Scientific Meeting. San Antonio USA November 7-11 1993 A. Niveleau, C. Reynaud and D. Shuker. Urinary modified nucleosides in cancer patients receiving combinbation chemotherapy including methylating agents. Interactions of cancer susceptibility genes and environmental carcinogenesis AACR IARC Meeting November 9-13, 1993 Lyon France L. Frappart, C. Reynaud and A. Niveleau. Nucléosides modifiés. Immunodétection in situ. Application au cancer du sein. Journées de Pathologie. SFANP. 8-9 Décembre 1993 Paris. A. Barbin, A. Niveleau, B. Dutrillaux and C.A. Bourgeois. In situ DNA methylation analysis of human tumor cells: mapping of methylcytosine-rich bands 4th European Workshop on Cytogenetics and Molecular Genetics of human solid tumors. April 23-26 1994 Amsterdam. A. Barbin, E. Delain, A. Niveleau, B. Malfoy,, B. Dutrillaux and C.A. Bourgeois. New developements in imunochemistry for in situ detection of DNA methylation in image cytometry. Third conference of the European Society for Analytical Cellular Pathology. May 1620 1994 Grenoble J. Bernardino, A. Niveleau, B. Malfoy, B. Dutrillaux and C.A. Bourgeois. In situ analysis of DNA methylation by immunofluorescence using digital imaging microscopy. Third conference of the European Society for Analytical Cellular Pathology. May 16-20 1994 Grenoble. M. Bensaada, L. Druart, V. Cacheux, C. Terré, A. Niveleau, G. Tachdjian. A rapid and simple method to analyze in situ the methylation pattern of chromosomes. 26 th Meeting of the European Society of Human Genetics. June 1-5 1994 Paris Bernardino,J., Malfoy,B., Dutrillaux,B., Niveleau,A. Bourgeois,C.A. 1995 X chromosome inactivation and DNA methylation, an in situ study. Colloque SBCFATIPE/CNRS Biologie du Développement Lyon. Habib,M., Reynaud,C., Souchier,C., Bella,C., Frappart,L. and Niveleau,A. Immunodetection in situ of mofified nucleosides. Application to cells grown in vitro, human breast tissues and peripheral blood lymphocytes. 12th International Conference on Human Tumor Markers New-York 11-14 Juin 1995 Bourgeois, C., Habib,M., Veilleux, C., Bernardino, J., Malfoy, B., Dutrillaux, B. Niveleau, A DNA methylation and cancer: in situ analysis.12th International Conference on Human Tumor Markers New-York 11-14 Juin 1995 Bourgeois,C., Habib,M., Bernardino,J., and Niveleau,A. DNA Methylation and Cancer: in situ analysis.XXIII Meeting of the International Society for Oncodevelopment, Biolgy and Medicine. Montreal 10-13 Septembre 1995 Habib, M., Reynaud, C. Souchier, C., Frappart, L. and Niveleau, A. The accessibility of 5-methyldeoxycytidine-rich regions in paraffin-embedded human breast tissues. Fifth International Congress Hormones and Cancer Québec 16-20 Septembre 1995 Habib, M. Bella,C. and Niveleau, A. DNA methylation: a flow cytometry study 2° Congrès de l'Association Française de Cytometrie Reims 27-29 Septembre 1995 de Capoa, A., Menendez, F., Poggesi, I., Giancotti, P., Marotta, M.R., Niveleau, A., Archidiacono, N., Rocchi, M. In situ hybridization of β satellite DNA probes and anti-5Mec antibodies binding on human chromosomes: image analysis. 8° Congrès annuel de l’Association des cytogénéticiens de langue française Clermont-Ferrand 20-21 Septembre 1995 Chiesa, J., Chateau,M.T., Ginestier Verne,C., Henry,L., Niveleau,A. and Bureau, J.P. Etablissement du profil de méthylation du génome humain par analyse in situ de chromosomes metaphasiques 8° Congrès annuel de l’Association des cytogénéticiens de langue française Clermont-Ferrand 20-21 Septembre 1995 de Capoa, A., Menedez, F., Poggesi, I., Biancotti, P., Grappelli, C., Marotta, M.R., Miveleau, A., Reynaud, C., Archidiacono, N., Rocchi, M. Labelling by anti-5MeC antibodies as a measure of the methylation status of human constitutive heterochromatin.1995 Chrom Res, 3 suppl 1, 45. 6Reynaud, C., Sasco, A., Souchier, C., Frappart, L., Niveleau, A. 1996 Hypomethylation of DNA and excretion of modified nucleosides in breast cancer. An immunochemcal study. 19th Annual San Antonio Breast Cancer Symposium. San Antonio Texas USA. December 12-14. Sasco, A., Rey, F., Reyanud, C., Bobin,.Y., Clavel,M., Niveleau,A. 1996 Breast cancer prognostic significance of some modified nucleosides excreted in urine. Predictive Oncloogy and Therapy 3rd International symposium. Nice. October 26-28. Benchaib,M., Ajina, M., Souchier, C., Niveleau,A., Lornage, J. and Guérin, J.F. 1997 A protocol to investigate DNA methylation staining by image analysis in human spermatozoa. International Symposium on Genetics of Human Male Fertiity. Toulouse Juillet Habib, M., Niveleau,A., de Capoa, A., Grappelli, C., Giaretti, W., Da Vinci, M. 1997 DNA methylation in normal and transformed cells: an in situ immunochemical study. 4th New England Biolabs Workshop on Biological DNA Modifications. Innsbruck-Igs, September 3-7. Habib, M., Fares, F., Drouet, E., Niveleau, A. 1998 DNA methyltransferase activity and DNA methylation levels: evaluation with a non-radioactive technique. 89 th Annual Meeting AACR. March 28-April 1. New-Orleans USA M. Habib, F. Fares, C.A. Bourgeois, C. Bella, J. Bernardino, F. Blazquez-Hernandez , A. de Capoa , A. Niveleau 1998 HYPOMETHYLATION OF DNA IN EBV-TRANSFORMED CELLS Curie Workshop on epigenetics and DNA methylation Paris September, 23-26. Hernandez-Blazquez, F. J M. Habib, J.M. Dumollard, M. Benchaib and A. Niveleau VISUALIZATION AND EVALUATION OF GLOBAL DNA HYPOMETHYLATION IN HUMAN COLON CANCER TISSUE SECTIONS BY IMMUNOHISTOCHEMICAL STAINING AND IMAGE ANALYSIS Curie Workshop on epigenetics and DNA methylation Paris September, 23-26. M. Habib, F. Fares, C.A. Bourgeois, C. Bella, J. Bernardino, F. Blazquez-Hernandez , A. de Capoa , A. Niveleau 1998 HYPOMETHYLATION OF DNA IN EBV-TRANSFORMED CELLS Curie Workshop on epigenetics and DNA methylation Paris September, 23-26. de Capoa, A., Grappelli, C, Caiafa, P.,Zardo, G., Cafolla, A., Niveleau, A. 1999 Anti-5-methylcytosine antibodies as a tool for detecting in mammalian cells spontaneous or induced DNA hypo- and hypermethylation associated with chromatin compaction. Second European Genetics Conference July 3-6, Vienna Austria F. Hernandez-Blazquez, M. Habib; J.-M. Dumollard, L. Frappart, E. Drouet, A. de Capoa, A. Niveleau. 2000 DNA HYPOMETHYLATION IN CANCER CELLS AND TISSUES: VISUALIZATION AND COMPUTER-ASSISTED ASSESMENT BY IMMUNOCHEMISTRY AACR annual meeting. San Francisco, 1-5 Avril A. de Capoa, L. Frappart, J.M. Dumollard, F.J. Hernandez-Blazquez, M. Habib and A. Niveleau. 2000. DNA methylation status in cancer cells and tissues: the immunochemical approach. Cancer Detection and Prevention. 5th International Symposium on Predictive Oncology and Therapy. Genève 28-31 Octobre. C.J. Piyathilake, AR Frost, WC Bell, H Weiis, D. Oelschlager, GL Johanning, A. Niveleau, DC Heimburger, W.E. Grizzle. 2001 Altered gloal methylation of DNA: an epigenetic differencein susceptibility for lung cancer is associated with its progression. 25th Annual Meeting of the American Society of Preventive Oncology. 11-13 Mars New-York.N.Y. C.J. Piyathilake, O. Henao, M. Macaluso, AR Frost, GL Johanning, WC Bell, DC Heimburger, A. Niveleau, , W.E. Grizzle 2001 Race and age-dependent alteration in global methylation of DNA in squamous cell carcinoma of the lung. FASEB meeting March 30th -April 4th. Orlando Fl.