Shimadzu CLASS-VP™ Validation and Productivity for Chromatography — Made Easy! CLASS-VP™ — A New Program With New Tools, To Make Chromatographic Data Processing More Powerful Than Ever. For years, you have relied on Shimadzu to provide the hardware and software to run your chromatography experiments quickly, easily and reliably. Now with the unification of Shimadzu’s CLASS (Chromatography Laboratory Automation Systems Software) and EZChrom, you can take advantage of Shimadzu’s proven leadership and experience, along with new tools to enhance productivity and validate your processes and results. CLASS-VP™ is a multi-tasking chromatography data acquisition and instrument control system operating under Microsoft ® Windows™. Like EZChrom, it may be used to take control of to four chromatographic systems at the same time, either GC or LC or both, adding the capability to evaluate results or criteria before or after the acquisition of sample data in a batch. Powerful New Features. CLASS-VP™ offers new calibration types and integration events, including Shimadzu’s Chromatopac integration algorithm. The software provides numerous new GLP features, such as storage of results with data and method, file overwrite protection and intelligent batch table with decision-making capabilities. Batch Capabilities which enhance productivity by automating sampling, QC and calibration routines have been greatly expanded for the release of CLASS-VP™. The new Sample Descriptor File allows you to identify samples with up to 64,000 characters. Productivity and validation are enhanced by the System Suitability capabilities of CLASS-VP™, which allow you to make sure the system is running properly before samples are run. Failure action, using Batch Table under System Suitability, proves the system is correctly calibrated, both for immediate use and future validation. New QC Check Standards run under peak and batch table, allowing continuous checking of calibration. Calibration Functionality allows identification and calibration for each compound independently within a single run. Enhanced System Controls are also part of new CLASS-VP™, including the first automated LC Fraction collector, an Advanced Pretreatment Routine to customize HPLC injections on Shimadzu’s autosamplers, and tabbed GC controls. CLASS-VP™ has been designed to work with the hardware you already own. Expanded third-party System Support includes HP 5890 GC, HP 7673A autosampler and PE Nelson interface. throughout a PC network without additional protocols.. Your LC, GC And PC Have Been Waiting For Shimadzu CLASS-VP™. Shimadzu CLASS-VP™ gives you unprecedented control over all your chromatography data. If your lab uses either HPLC or GC or both, Shimadzu CLASS-VP™ will supercharge everything from methods development to batch reprocessing. With just a few clicks of a mouse, powerful Windows™-based Shimadzu CLASS-VP™ will automate the entire process of configuring, developing, running, reprocessing and validating your experiment. To get started, simply enter runtime and sampling rate. It’s that easy. And your data acquisition can run in the background, freeing up Shimadzu CLASS-VP™ to begin another experiment — or to work on your PC in other Windows™-based software. With Shimadzu SuperCompare™, you can view up to 32 overlaid chromatograms or PDA spectra. With data acquisition complete, modify parameters directly within the chromatogram, either graphically (using the easy-to-use toolbar) or from the keyboard for utmost flexibility. Change a concentration; change a parameter; even change a sequence — and Shimadzu CLASS-VP™ lets you choose to make the change in real time or off-line. File management in CLASS-VP™ is easy and intuitive. Name your file as you need it, and add comments to help you identify file contents on the fly. You can even see a small graphic of your chromatogram in your file menu. Store your Methods and Results with the raw data. With Shimadzu CLASS-VP™, you don’t need to be a programmer to get the most out of the software. GLP is as easy as saving a document. Shimadzu CLASS-VP™ sets you free — to concentrate on science. Use CLASS-VP™ To Control Either LC Or GC — Or Both, Simultaneously. Shimadzu CLASS-VP™ is a software package designed for scientists by scientists who demanded a fully functional, do-everything program. As a result, Shimadzu CLASSVP™ was developed jointly on GC and LC platforms, working equally well on both. Shimadzu CLASS-VP™ allows definition or modification of detectors, pumps, valves or autosamplers — with the click of a mouse, if you like. In fact, Shimadzu CLASS-VP™ offers complete data acquisition and processing for any chromatography system, from any manufacturer, with analog output. In other words, just about everything. And it will process four signals from each of four different instruments at the same time. CLASS-VP™ for HPLC Shimadzu CLASS-VP™ truly shines when connected to Shimadzu chromatography systems. With Shimadzu’s state-of-the-art LC-10A HPLC system, Shimadzu CLASSVP™ offers expanded control of preparative pumps and intelligent fraction collectors, ideally suited to purification and isolation. CLASS-VP™ has been designed to also be compatible with earlier generations of Shimadzu HPLC systems, including the SCL-6B and LPI-6B. CLASS-VP™ enhances Shimadzu’s leadership position in automation solutions. New capabilities include the ability to integrate intelligent fraction collection and robotic sample pretreatment. The high-powered graphical interface of CLASS-VP™ allows users to identify sample and sample status by color, including identification of current sample, standards, reagent addition targets or sample spiking targets. Through its interface to the LC System Controller, the FRC-10A Intelligent Fraction Collector is controlled to provide intelligent peak picking and even collection on the basis of peak purity, adding an entire new dimension to fraction collection. For example, these new capabilities allow users to combine purity confirmation using a photodiode array with targeted fraction collection, and get visual confirmation of exactly which portion of a peak was collected, and in what vessel. CLASS-VP™ allows researchers to fully exploit the analytical, micro and biocompatible sampling capabilities of Shimadzu SIL-10A, SIL-10Axl and the SIL-10Ai robotic autosamplers. A simple script language allows user-customizable sample pretreatment that allows: • Sample spiking • Sample dilution • Reagent addition and derivatization The family of Shimadzu HPLC autosamplers provide robotic functionality for the most demanding applications. This allows the user to be as creative as possible in performing pre-column derivatizations or sample treatment without overwhelming a routine or occasional user with unwanted details. CLASS-VP for GC Shimadzu CLASS-VP™ is also a stellar performer in conjunction with Shimadzu GCs. The CLASS-VP GC control package provides automated graphical control over both the GC and its autosampler, including complete random sample access. The software automatically calculates acceptable values in method development, and automatically rejects invalid parameters. Shimadzu CLASS-VP™ is totally compatible with Shimadzu GC-17, GC-14 and GC-15A Gas Chromatographs. GCs can be configured automatically by the software and include critical compliance information such as instrument ROM version, injectors and detectors present. GC control completely supports all GC-17 Advanced Flow Control (AFC) functionality including determination of constant linear velocity pressure programs, (which minimize sample run times without sacrificing chromatographic integrity) to provide the ultimate in productivity tools. All GC events are also supported and spelled out, including those for carrier gas conservation, such as split ratio reduction, detector signal zeroing and external valve control. Intuitive operations are ensured for Shimadzu GC Automatic Injection Systems including the AOC -14, AOC-17 and AOC-1400 which utilize our intelligent batch functionality to control the autosampler in a decision-making process. Failure criteria such as meeting a concentration or a % Relative Standard Deviation (RSD) minimum for a standard sample, can be utilized in a fashion that will provide the re-injection of the sample by the AOC. Overall, this means that complete automated control of the GC processes are available from one location in an intuitive user interface. GC control is not limited to Shimadzu GCs. Control modules for the HP 5890 and HP 7673 autosampler are also available. Operations are virtually identical to those in Shimadzu GC control, minimizing the learning curve. Manual flow control and EPC controlled versions of the HP 5890 are supported, including setting of pressure and flow programs. Failure actions can be used in conjunction with the HP 7673 autosampler also, maximizing its productivity. The PDA Control Features of CLASS-VP™ Allow The Most Complete Spectrum of Detection CLASS-VP™ is the ideal tool to take control of any HPLC detector, especially Shimadzu SPD-M10A and SPD-M10AV Photodiode Array (PDA) detectors. CLASS-VP™ turns reams of data into easy-to-understand results, quickly and intuitively. And Shimadzu has now added new tools to enhance PDA control. An enhanced purity algorithm for PDA now allows a more complete analysis of component homogeneity by analyzing all spectra beneath the chromatographic peak. In addition, the new analog output option for PDA affords the convenience of data output to previously validated data stations. These features are designed to provide comprehensive purity determination and the convenience of connectivity to existing validated systems. With the CLASS-VP™ PDA module, the Windows™ interface zips you to an easy-tounderstand interface built on icons, which guide you effortlessly through method development, instrument control and report formatting. It also puts key functions, including Snapshot, Freeze Frame and on-the-fly spectra display right at your fingertips. With CLASS-VP™ and Shimadzu PDA, you are ready for peak purity determination, library searches for spectra and derivatives, multi-channel analysis and extensive GLP documentation. With CLASS-VP™, you can view spectral plots in real time. The exclusive On-Demand integration feature allows you to view a contour plot even before analysis is complete and you can begin using the results. You can begin immediately building a library of spectra, which can be used in library searches (either graphically or numerically). CLASS-VP™ allows you to view your data in a variety of ways. Choose from a Gallery of different views for a ratio plot, a fully rotatable 3D plot, multi-chromatograms — or combinations of views. And all of these views are fully compatible with custom reporting, so that what you see is truly what you get. PDA software is available as an option. How Good Laboratory Practice Became Practical. No other software package provides a more robust set of Integration, Quantitation Analysis and Mathematical Functions for chromatography. And no other chromatography software goes to such lengths to assure that your experiments are GLP/GaLP-compliant. Shimadzu CLASS-VP™ stores method and results — in the same file as the chromatogram, and prevents over-writing of data files. It also stores method development and changes — automatically. Shimadzu CLASS-VP™ tracks all modifications and errors and can be used for automatic calibration. Standard Deviation and Relative Standard Deviation calculations are the same methodology as used by the U.S. Environmental Protection Agency. New System Suitability Reporting capabilities in Shimadzu CLASS-VP™ validate the performance of your instruments and method. System Suitability capabilities within Shimadzu CLASS-VP™ are fully compliant with FDA requirements, and conform to ASTM, USP and BP methods. System security has also been enhanced in CLASS-VP™. In addition to System Administrator, Methods Developer and Operator user levels — each with its own set of system privileges and controls — Shimadzu CLASS-VP™ offers log-in security by module, isolating the security of each function from every other. CLASS-VP™ has its own log files for complete audit trails. Change a concentration — and record it. Increase runtime — Shimadzu CLASS-VP™ helps you to remember. Recalibrate an entire series — and make it part of the permanent file. Reliable. Verifiable. Comprehensive. Shimadzu CLASS-VP™. You Can Use The Report Formats We Provide — Or Easily Create Just What You Need. We don’t expect you to output your work the way we want it…your reports need to contain just the information you need. That’s why Shimadzu CLASS-VP™ allows you to select up to four chromatograms and four reports per page, and decide where information will be placed on a page. Select, edit and place: • Peak Number • Peak Begin Time • Peak End Time • Retention Time • Width • USP Width • Width at 5%, 10%, 50% • Concentrations • Integration Codes • Response Factor • Relative Retention Time • Name, Area, Height • Area %, Height % • Theoretical Plates • Resolution • Peak Asymmetry • Peak Group Amounts Peaks are numbered and also defined with user-selectable names. You name your method and include your own sample ID, acquisition time and date, and annotation to meet your specific needs. Once you’ve seen it on the monitor, true WYSIWYG (What printed. When you need even more reporting flexibility, use the Shimadzu CLASS-VP™ Dynamic Data Exchange (DDE) to export data directly into any other Windows™-based program, or use the Custom Export feature to create an ASCII file. Shimadzu CLASSVP™ also offers Custom Importing features, and can import or export using AIA file format. (GPC copy to come from John Brady) The CLASS-VP Gel Permeation Chromatography (GPC) module performs molecular weight determination and characterization using narrow or broad standards calibration — with or without universal calibration. Collect and analyze chromatograms from multiple instruments, each with up to four detectors. Select linear, quadratic, or cubic curve fits, and calculate the full range of molecular weight distribution values including numberaverage, weight-average, Z-average, Z+1-average, Z/weight-average, Z+1/weightaverage, viscosity average, intrinsic viscosity and polydispersity. This option also lets you customize your reports and stripcharts for publication-quality presentation. CLASS-VP’s mouse-driven graphics interface makes this GPC package easy to use, and provides flexibility for manual or automatic determination of calibration information, slice rejection criteria and baseline positioning. A user selects the optimum slice width, as well as independent baseline and processing start/end points. And since the GPC software is fully integrated into CLASS-VP, each user can individually choose their own degree of automation or manual control. When You Order Shimadzu CLASS-VP™, It Comes In Just Your Size. provides a toolset that includes: • Automatic batch reprocessing • Graphic or keyboard entry of integration events - peak width - threshold - shoulder sensitivity - define peaks - define groups - integration off - valley-to-valley baseline - horizontal baseline - tangent skim - minimum area - negative peak integration - manual baseline - manual peak insertion - sampling frequency • Graphic or keyboard Peak Table and grouping entry • Quantitation via % area, % height, normalization to %, external standard or internal standard • Single point linear cubic or quadratic and exponential calibration fits with optional forced origin for up to 16 levels • Scaling operations 1/x, 1/x2, log[x] and 1/log [x] • User defined replicates for each level with accompanying statistics • Deselect outliers and replicate, if desired • Bracket unknowns with calibration standards or use previous calibration response factors • Graphical Calibration Curve Peak Library • Custom reports and internal standard, external standard and standard area % reports • Grouping and Calibration reports • Mathematical functions, including subtraction, addition, multiplication, division and first and second derivatives of chromatograms, storing altered files • User selectable number of named peaks If you need just the reprocessing capabilities, Shimadzu CLASS-VP™ is available as Shimadzu CLASS-VP™ Lite. Shimadzu CLASS-VP™ is also available in a Starter package, suitable for control of a single chromatograph with a single detector. Starter provides the full functionality of Shimadzu CLASS-VP™, and can be field upgraded to a complete version with the addition of A/D interfaces, SuperCompare, User Log-In and optional instrument control packages. can be configured for control and acquisition of up to four instruments with four detectors each at the same time. This version of Shimadzu CLASS-VP™ is also available with either Shimadzu CLASS-VP™ HPLC or Shimadzu CLASS-VP™ GC, or both custom liquid or gas chromatography control packages. PDA control software is also available as an option. Finally, Shimadzu CLASS-VP™ is now available in a network version that is “NetworkAware.” Installation of this software on a network will make the experiments run on its PC available throughout a network, without additional protocols, making the most of your existing investment in computers, workstations, printers, plotters and LIMS. We’ve taken validation to the very limit — because you asked for it! CLASS-VP™ has been developed to provide the ultimate in confidence — in the lab, and in review. As a fist step, the software itself is validated and tested against NIST standards and is guaranteed to meet — or exceed specifications. In addition, any boardware or interface that ships with CLASS-VP™ is similarly warranted. These validations and warranties are fully documented and supported by Shimadzu Scientific Instruments, Inc. In addition, your routines and experiments run with CLASS-VP™ are fully validated as you work. Method development is validated in-progress, along with system performance, under the CLASS-VP™ System Suitability capabilities. If either system functionality or method development do not conform to standards, CLASS-VP™ will let you know — immediately and automatically. “Smart” batch functions in CLASS-VP™ validate results and automate the process of reanalysis while keeping track of your work. CLASS-VP™ also contains the latest advances in software development and document management to provide a complete audit trail — without the need for operator involvement or decision-making. When you are done for the day, your methods and changes are automatically stored with your results. CLASS-VP™ software, another reliable, standard-setting solution for science from Shimadzu.